(r2) Barrick Lab :: ProtocolsRecipesM9

---+++ M9 Minimal Media Plates

|  *1L*  |  *Component* |
| 6 g | Sodium phosphate, Na<sub>2</sub>HPO<sub>4 (anhydrous)</sub>  |
| 3 g | Potassium phosphate, KH<sub>2</sub>PO<sub>4</sub> |
| 0.5 g | Sodium chloride, NaCl |
| 1 g | Ammonium chloride, NH<sub>4</sub>Cl |
| 16 g | Agar |
| 1 ml | Antifoam |
 Add dH<sub>2</sub>O to 1 liter. Autoclave.

After media is autoclaved add the following sterile ingredients:
|  *1L*  |  *Component* |
| 1 ml | 1M Magnesium sulfate, MgSO<sub>4</sub> |
| 1 ml | 0.1M Calcium chloride CaCl<sub>2</sub> |
| 10 ml | 10% (w/v) glucose (or other carbon source)  |