DM: Davis Minimal

7 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to 1 liter. Autoclave. Then add
1 ml 10% Magnesium Sulfate MgSO4 MW 120.37 Note* The MgSO4 must be autoclaved separately.
1 ml 0.2% Thiamin (vitamin B1)  

Final nutrient concentrations:

67.4 mM Phosphate (PO43-)
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
0.83 mM Magnesium (MGgsup>2+)
1.7 mM Citrate
139 µM Glucose (in DM25)
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 Barrick Lab  >  ProtocolsMediaRecipes  >  ProtocolsRecipesDavisMingioli

Topic revision: r2 - 2011-07-19 - 21:57:36 - Main.BrianRenda
 

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