Czapek Broth (CB)

Combine in a flask:

1. In a flask, combine sodium nitrate, potassium phosphate dibasic, potassium chloride, and ferrous sulfate heptahydrate in diH₂O
   • If making 500 mL of media, use 995.85 mL diH₂O
   • If making 1 L of media, use 497.925 mL diH₂O
2. Once reagents are combined and dissolved, measure pH and adjust until it reaches 7.5
3. Once pH of 7.5 is achieved, autoclave the solution and allow to cool
4. Once cooled, add 1 M magnesium sulfate

Source: Throne-Holst et. al 2007