Barrick Lab :: ProtocolsRecipesCzapekBroth

---+++ Czapek Broth (CB)

Combine in a flask:
|*500 mL* | *1 L*  |  *Component*  |
| 1.5 g | 3 g | sodium nitrate | 
| 0.5 g | 1 g | potassium phosphate dibasic |
| 0.25 g | 0.5 g | potassium chloride | 
| 9 mg | 18 mg | ferrous sulfate heptahydrate | 
| 2.075 mL | 4.15 mL | 1 M sterile magnesium sulfate| 

   1. In a flask, combine sodium nitrate, potassium phosphate dibasic, potassium chloride, and ferrous sulfate heptahydrate in diH<sub>2</sub>O
      * If making 1 L of media, use 995.85 mL diH<sub>2</sub>O
      * If making 500 mL of media, use 497.925 mL diH<sub>2</sub>O
   2. Once reagents are combined and dissolved, measure pH and adjust until it reaches 7.5
   3. Once pH of 7.5 is achieved, autoclave the solution and allow to cool
   4. Once cooled, add 1 M magnesium sulfate


Source: [[https://doi.org/10.1128/AEM.00064-07][Throne-Holst et. al 2007]]

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Topic revision: r2 - 2024-10-16 - 02:49:56 - Main.MeghnaVergis