ABMS: Acinetobacter Minimal Succinate

Courtesy of the Averhoff lab, with modifications for available reagents.

To make standard ABMS, combine the following sterilized ingredients at room temperature:

To make ABMS plates:

Add 16g/L agar and 1mL/L antifoam to diH₂O, then autoclave in a 2L flask. When water and agar are cool enough to touch, add component solutions. Optional: warm components at 55C in a water bath before combining with agar to reduce the temperature difference when combined.

ABMS Component Recipes

SL9 (trace minerals)

Add 1mL of 10mL 10X solution:

Bring to 1000mL and pH 6.5. Note: this requires a lot of NaOH, so leave room when bringing up to volume.

Mineral Solution (20X)

Bring to 500mL in a graduated cylinder with deionized water.

Phosphate Buffer (20X)

Bring to 500mL.