Barrick Lab :: ProtocolsRecipes1416,4-hydroxybenzoicacidmedium

1L	4L	Component
4.25 g	17.0 g	Potassium Phosphate (dibasic) K₂HPO₄ trihydrate
1.00 g	4.00 g	Sodium Phosphate (monobasic) NaH₂PO₄ monohydrate
2.00 g	8.00 g	Ammonium chloride NH₄Cl
0.20 g	0.40 g	Magnesium Sulfate MgSO₄ heptahydrate
12.0 mg	48.0 mg	Ferrous Sulfate FeSO₄ heptahydrate
3.00 mg	12.0 mg	Manganese Sulfate MnSO₄ monohydrate
3.00 mg	12.0 mg	Zinc Sulfate ZnSO₄ heptahydrate
1.00 mg	4.00 mg	Cobalt (II) Sulfate CoSO₄
0.10 g	0.40 g	Nitrilotriacetic acid *
1.00 g	4.00 g	4-Hydroxybenzoic acid *

* Dissolve these components in water made alkaline with concentrated NaOH before adding other components.

Adjust medium for final pH 7.2, +/- 0.2. Autoclave for at least 15 minutes.

If plates needed, add 15.0 g of agar per liter to the above recipe.

Barrick Lab > ProtocolsMediaRecipes > ProtocolsRecipes1416,4-hydroxybenzoicacidmedium

Contributors to this topic

CraigBarnhart

Topic revision: r1 - 2013-06-24 - 17:50:39 - Main.CraigBarnhart