1416: 4-hydroxybenzoic acid medium (for JJ-1b, Bacillus sp.)
| 1L |
4L |
Component |
| 4.25 g |
17.0 g |
Potassium Phosphate (dibasic) K2HPO4 trihydrate |
| 1.00 g |
4.00 g |
Sodium Phosphate (monobasic) NaH2PO4 monohydrate |
| 2.00 g |
8.00 g |
Ammonium chloride NH4Cl |
| 0.20 g |
0.40 g |
Magnesium Sulfate MgSO4 heptahydrate |
| 12.0 mg |
48.0 mg |
Ferrous Sulfate FeSO4 heptahydrate |
| 3.00 mg |
12.0 mg |
Manganese Sulfate MnSO4 monohydrate |
| 3.00 mg |
12.0 mg |
Zinc Sulfate ZnSO4 heptahydrate |
| 1.00 mg |
4.00 mg |
Cobalt (II) Sulfate CoSO4 |
| 0.10 g |
0.40 g |
Nitrilotriacetic acid * |
| 1.00 g |
4.00 g |
4-Hydroxybenzoic acid * |
* Dissolve these components in water made alkaline with concentrated NaOH before adding other components.
Adjust medium for final pH 7.2, +/- 0.2. Autoclave for at least 15 minutes.
If plates needed, add 15.0 g of agar per liter to the above recipe.