(r6) Barrick Lab :: ProtocolHowTo

---+!! How to Create a Protocol

%TOC%

---++ Tips for Design

 You should generally organize a protocol to have sections that are relevant from this list:
   * Supplies (materials, strains, primers)
   * Step-by-step instructions for each step
   * Expected results (quantitative information, graphics, images)
   * Common problems and troubleshooting
   * Spreadsheets for calculations

Include instructions that reference specific reagents (strains, primers, etc.) for performing *positive* and *negative* control reactions. It is very important and can save a great deal of time if you run these reactions to diagnose bad reagents or a general problem with carrying out the protocol versus a problem with applying it in a specific case.

*These protocols are public, so don't post passwords, protected information, or copyrighted material. Use the [[InternalList][internal Barrick Lab web pages]] for that.*

---++ How to Create a New Protocol / Wiki Page

   1 Navigate to the page that will link to your new page.
   1 Edit this page.
      * Add a link using this syntax: <verbatim>[[TopicName][Link Text]]</verbatim>
      * =TopicName= should be a [[TWiki.WikiWord][WikiWord]] that is descriptive of the new topic. Many topics on the Barrick Lab pages begin with certain words indicating how they are categorized, e.g. "Protocol", "Reference", "Internal".
      * =Link Text= will be the text of the hyperlink that appears on the web page.
      * Save your edit.
   1 Now, a red link to your new page should appear when you view this page. 
   1 Click on the red link and you will be taken to the edit window for your new page!

---++ How to Add Images

   1 Click the "attach" link at the bottom of your page
   1 Choose your image file:
      * Avoid spaces in the file name. Use underscores instead.
      * Under "Properties" check the box called "Create a link to the attached file."
   1 After you click "Upload file" your image will appear at the bottom of the page after a bullet point.
   1 Now edit the page and move the =<img>= tag to where you want it displayed in the page.

Tips:
   * If you accidentally forget to hide the attachment, you can click on the attach link and then edit the properties of the file you uploaded at any later time. 
   * You can refer to an image that is attached to a different page with this syntax: <verbatim> <img src="%PUBURL%/%WEB%/OtherTopic/image.jpg"> </verbatim>

---++ Further Tips for Editing the Wiki

Try editing a page where you see what you want to do and copying the syntax!! Monkey see, Monkey do.

Or, you could read the [[TWiki.TWikiReferenceManual][TWiki Reference Manual]]. (Useful sections: [[TWiki.TextFormattingRules][Shorthand]] | [[TWiki.TWikiVariables][Variables]] | [[TWiki.TWikiDocGraphics][Icons]])

Attachments

Topic attachments
I	Attachment	History	Action	Size	Date	Who	Comment
jpg	Figure_GoldenGateTransformation.JPG	r1	manage	25.1 K	2016-04-07 - 19:33	GabrielSuarez	Reaction components: pYTK001-tdk-kan Entry Vector + 5'-flank + 3'-flank

Barrick Lab > ProtocolList > ProtocolHowTo

Contributors to this topic

JeffreyBarrick, GabrielSuarez, AnthonyVandieren, DanielDeatherage, BrianRenda

Topic revision: r6 - 2016-04-07 - 19:33:18 - Main.GabrielSuarez