Difference: ProtocolHowTo (1 vs. 9)

Revision 92020-12-07 - JeffreyBarrick

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META TOPICPARENT name="ProtocolList"

How to Create a Protocol

Revision 82020-11-22 - JeffreyBarrick

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META TOPICPARENT name="ProtocolList"

How to Create a Protocol

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  Try editing a page where you see what you want to do and copying the syntax!! Monkey see, Monkey do.
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Or, you could read the TWiki Reference Manual. (Useful sections: Shorthand | Variables | Icons)
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General resources

Useful links for editing the Barrick Wiki (required log in)

Revision 72017-12-14 - JeffreyBarrick

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META TOPICPARENT name="ProtocolList"

How to Create a Protocol

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 Try editing a page where you see what you want to do and copying the syntax!! Monkey see, Monkey do.

Or, you could read the TWiki Reference Manual. (Useful sections: Shorthand | Variables | Icons)

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META FILEATTACHMENT attachment="Figure_GoldenGateTransformation.JPG" attr="" comment="Reaction components: pYTK001-tdk-kan Entry Vector + 5'-flank + 3'-flank" date="1460057598" name="Figure_GoldenGateTransformation.JPG" path="Figure_GoldenGateTransformation.JPG" size="25750" stream="Figure_GoldenGateTransformation.JPG" tmpFilename="/usr/tmp/CGItemp36285" user="GabrielSuarez" version="1"

Revision 62016-04-07 - GabrielSuarez

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META TOPICPARENT name="ProtocolList"

How to Create a Protocol

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 Try editing a page where you see what you want to do and copying the syntax!! Monkey see, Monkey do.

Or, you could read the TWiki Reference Manual. (Useful sections: Shorthand | Variables | Icons)

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META FILEATTACHMENT attachment="Figure_GoldenGateTransformation.JPG" attr="" comment="Reaction components: pYTK001-tdk-kan Entry Vector + 5'-flank + 3'-flank" date="1460057598" name="Figure_GoldenGateTransformation.JPG" path="Figure_GoldenGateTransformation.JPG" size="25750" stream="Figure_GoldenGateTransformation.JPG" tmpFilename="/usr/tmp/CGItemp36285" user="GabrielSuarez" version="1"

Revision 52016-03-24 - JeffreyBarrick

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META TOPICPARENT name="ProtocolList"
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How to Create a Protocol

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How to Create a Protocol

 

Tips for Design

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  Include instructions that reference specific reagents (strains, primers, etc.) for performing positive and negative control reactions. It is very important and can save a great deal of time if you run these reactions to diagnose bad reagents or a general problem with carrying out the protocol versus a problem with applying it in a specific case.
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These protocols are public, so don't post passwords, protected information, or copyrighted material. Use the internal Barrick Lab web pages for that.
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These protocols are public, so don't post passwords, protected information, or copyrighted material. Use the internal Barrick Lab web pages for that.

How to Create a New Protocol / Wiki Page

  1. Navigate to the page that will link to your new page.
  2. Edit this page.
    • Add a link using this syntax:
      [[TopicName][Link Text]]
    • TopicName should be a WikiWord that is descriptive of the new topic. Many topics on the Barrick Lab pages begin with certain words indicating how they are categorized, e.g. "Protocol", "Reference", "Internal".
    • Link Text will be the text of the hyperlink that appears on the web page.
    • Save your edit.
  3. Now, a red link to your new page should appear when you view this page.
  4. Click on the red link and you will be taken to the edit window for your new page!
 

How to Add Images

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  1. Click the "attach" link at the bottom of your protocol page
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  1. Click the "attach" link at the bottom of your page
 
  1. Choose your image file:
    • Avoid spaces in the file name. Use underscores instead.
    • Under "Properties" check the box called "Create a link to the attached file."
  2. After you click "Upload file" your image will appear at the bottom of the page after a bullet point.
  3. Now edit the page and move the tag to where you want it displayed in the page.
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Tips for Editing the Wiki

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Tips:
  • If you accidentally forget to hide the attachment, you can click on the attach link and then edit the properties of the file you uploaded at any later time.
  • You can refer to an image that is attached to a different page with this syntax:
     <img src="%PUBURL%/%WEB%/OtherTopic/image.jpg"> 

Further Tips for Editing the Wiki

Try editing a page where you see what you want to do and copying the syntax!! Monkey see, Monkey do.

 
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  • Edit other protocol pages to see how to do things in Wiki syntax and make the formatting or your protocol consistent with others.
  • We will eventually add some specific tags to make formatting references and contributors easier...
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Or, you could read the TWiki Reference Manual. (Useful sections: Shorthand | Variables | Icons)

Revision 42014-05-23 - JeffreyBarrick

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META TOPICPARENT name="ProtocolList"

How to Create a Protocol

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  You should generally organize a protocol to have sections that are relevant from this list:
  • Supplies (materials, strains, primers)
  • Step-by-step instructions for each step
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  • Expected results
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  • Expected results (quantitative information, graphics, images)
 
  • Common problems and troubleshooting
  • Spreadsheets for calculations
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Include instructions that reference specific reagents (strains, primers, etc.) for performing positive and negative control reactions. This can be used to diagnose bad reagents or a problem with carrying out the protocol versus a problem with applying it in a specific case.
>
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Include instructions that reference specific reagents (strains, primers, etc.) for performing positive and negative control reactions. It is very important and can save a great deal of time if you run these reactions to diagnose bad reagents or a general problem with carrying out the protocol versus a problem with applying it in a specific case.
 
Changed:
<
<
These protocols are public, so don't post passwords or protected information. Use the internal Barrick Lab web pages for that.
>
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These protocols are public, so don't post passwords, protected information, or copyrighted material. Use the internal Barrick Lab web pages for that.
 

How to Add Images

Changed:
<
<
  1. Click the "attach" ink at the bottom of your protocol page
>
>
  1. Click the "attach" link at the bottom of your protocol page
 
  1. Choose your image file:
Changed:
<
<
    • It's best to avoid spaces in the file name. Use underscores instead.
>
>
    • Avoid spaces in the file name. Use underscores instead.
 
    • Under "Properties" check the box called "Create a link to the attached file."
  1. After you click "Upload file" your image will appear at the bottom of the page after a bullet point.
  2. Now edit the page and move the tag to where you want it displayed in the page.

Tips for Editing the Wiki

Changed:
<
<
Will add some specific tags to make formatting references and contributors easier...
>
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  • Edit other protocol pages to see how to do things in Wiki syntax and make the formatting or your protocol consistent with others.
  • We will eventually add some specific tags to make formatting references and contributors easier...

Revision 32013-06-13 - BrianRenda

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META TOPICPARENT name="ProtocolList"

How to Create a Protocol

Revision 22013-06-13 - JeffreyBarrick

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META TOPICPARENT name="ProtocolList"

How to Create a Protocol

Line: 15 to 15
  These protocols are public, so don't post passwords or protected information. Use the internal Barrick Lab web pages for that.
Added:
>
>

How to Add Images

  1. Click the "attach" ink at the bottom of your protocol page
  2. Choose your image file:
    • It's best to avoid spaces in the file name. Use underscores instead.
    • Under "Properties" check the box called "Create a link to the attached file."
  3. After you click "Upload file" your image will appear at the bottom of the page after a bullet point.
  4. Now edit the page and move the tag to where you want it displayed in the page.
 

Tips for Editing the Wiki

Will add some specific tags to make formatting references and contributors easier... \ No newline at end of file

Revision 12012-08-07 - JeffreyBarrick

Line: 1 to 1
Added:
>
>
META TOPICPARENT name="ProtocolList"

How to Create a Protocol

Tips for Design

You should generally organize a protocol to have sections that are relevant from this list:

  • Supplies (materials, strains, primers)
  • Step-by-step instructions for each step
  • Expected results
  • Common problems and troubleshooting
  • Spreadsheets for calculations

Include instructions that reference specific reagents (strains, primers, etc.) for performing positive and negative control reactions. This can be used to diagnose bad reagents or a problem with carrying out the protocol versus a problem with applying it in a specific case.

These protocols are public, so don't post passwords or protected information. Use the internal Barrick Lab web pages for that.

Tips for Editing the Wiki

Will add some specific tags to make formatting references and contributors easier...

 
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