Barrick Lab :: Strain_side

---+ Commonly Used <em>E. coli </em>Strains

<a target="_blank" href="https://openwetware.org/wiki/E._coli_genotypes">OpenWetWare</a> offers a comprehensive repository of the genotypes of the most commonly used<em> E. coli </em>strains.

The following table points out some important features to take into account when working with some these _E. coli_ strains.

| *Strain* | *Genotype*  | *Observations* |
| _E. coli_ DH5&alpha; | F<sup>&ndash;</sup> _endA1_ _glnV44_ __thi-1__ _recA1_ _relA1_ _gyrA96_ _deoR_ _nupG_ _purB20_ &phi;80d<em>lacZ</em>&Delta;M15 &Delta;( _lacZYA-argF_)U169, hsdR17(<em>r<sub>K</sub></em><sup>&ndash;</sup><em>m<sub>K</sub></em><sup>+</sup>), &lambda;<sup>&ndash;</sup> | This strain is *auxotrophic for thiamine* ( _thi-1_) so that *requires supplementation* to be grown in minimal media such as DM. It has been observed that in minimum medium, even supplemented with thiamine (i.e. M9), this strain *grows slowly* compared to other strains such as BL21 and BW25113. |
| _E. coli_ TOP10 | F- mcrA &Delta;(mrr-hsdRMS-mcrBC) &phi;80lacZ&Delta;M15 &Delta;lacX74 nupG recA1 araD139 &Delta;(ara-leu)7697 galE15 galK16 rpsL(Str<sup>R</sup>) endA1 &lambda;<sup>-</sup> | |
| _E. coli_ BL21 | F<sup>&ndash;</sup> _ompT_ _gal_ _dcm_ _lon_ <em>hsdS<sub>B</sub></em>(<em>r<sub>B</sub></em><sup>&ndash;</sup><em>m<sub>B</sub></em><sup>&ndash;</sup>) [<em>malB</em><sup>+</sup>]<sub>K-12</sub>(&lambda;<sup>S</sup>) | |
| _E. coli_ BW25113 | <p><em>lacI</em><sup>+</sup><em>rrnB</em><sub>T14</sub> &Delta;<em>lacZ</em><sub>WJ16</sub> <em>hsdR</em>514 &Delta;<em>araBAD</em><sub>AH33</sub> &Delta;<em>rhaBAD</em><sub>LD78</sub> _rph-1_ <br />&Delta;<em>(araB&ndash;D)567</em> &Delta;<em>(rhaD&ndash;B)568</em> &Delta;<em>lacZ4787</em>(::<em>rrnB-3</em>) _hsdR514_ _rph-1_ </p> | |

-- Main.CamiloGomez - 14 Dec 2017
Barrick Lab > ProtocolList > Strain_side_notes
Contributors to this topic
CamiloGomez
Topic revision: r1 - 2017-12-14 - 22:04:00 - Main.CamiloGomez