---+ Commonly Used <em>E. coli </em>Strains <a target="_blank" href="https://openwetware.org/wiki/E._coli_genotypes">OpenWetWare</a> offers a comprehensive repository of the genotypes of the most commonly used<em> E. coli </em>strains. The following table points out some important features to take into account when working with some these _E. coli_ strains. | *Strain* | *Genotype* | *Observations* | | _E. coli_ DH5α | F<sup>–</sup> _endA1_ _glnV44_ __thi-1__ _recA1_ _relA1_ _gyrA96_ _deoR_ _nupG_ _purB20_ φ80d<em>lacZ</em>ΔM15 Δ( _lacZYA-argF_)U169, hsdR17(<em>r<sub>K</sub></em><sup>–</sup><em>m<sub>K</sub></em><sup>+</sup>), λ<sup>–</sup> | This strain is *auxotrophic for thiamine* ( _thi-1_) so that *requires supplementation* to be grown in minimal media such as DM. It has been observed that in minimum medium, even supplemented with thiamine (i.e. M9), this strain *grows slowly* compared to other strains such as BL21 and BW25113. | | _E. coli_ TOP10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(Str<sup>R</sup>) endA1 λ<sup>-</sup> | | | _E. coli_ BL21 | F<sup>–</sup> _ompT_ _gal_ _dcm_ _lon_ <em>hsdS<sub>B</sub></em>(<em>r<sub>B</sub></em><sup>–</sup><em>m<sub>B</sub></em><sup>–</sup>) [<em>malB</em><sup>+</sup>]<sub>K-12</sub>(λ<sup>S</sup>) | | | _E. coli_ BW25113 | <p><em>lacI</em><sup>+</sup><em>rrnB</em><sub>T14</sub> Δ<em>lacZ</em><sub>WJ16</sub> <em>hsdR</em>514 Δ<em>araBAD</em><sub>AH33</sub> Δ<em>rhaBAD</em><sub>LD78</sub> _rph-1_ <br />Δ<em>(araB–D)567</em> Δ<em>(rhaD–B)568</em> Δ<em>lacZ4787</em>(::<em>rrnB-3</em>) _hsdR514_ _rph-1_ </p> | | -- Main.CamiloGomez - 14 Dec 2017
This topic: Lab
>
WebHome
>
ProtocolList
>
Strain_side_notes
Topic revision: r1 - 2017-12-14 - CamiloGomez