How to select the right polymerase
Types of polymerase
Our lab carries 2 main types of polymerase:
Type |
Vender |
Cost per unit |
Product Information |
Image |
Taq DNA polymerase |
Syzygy Biotech |
0.18 |
link |
 Standard Taq |
Phusion High Fidelity DNA poly |
NEB |
0.86 |
Info about phusion |
 Phusion HF |
Applications
Phusion High Fidelity
High-fidelity PCR
The error rate of Phusion DNA Polymerase is 4.4 x 10^-7, which is industry-leading fidelity. Therefore, Phusion DNA Polymerase is suitable for all PCR applications requiring great accuracy.
Cloning
Phusion DNA Polymerase amplifies templates with an accuracy and speed previously unattainable with a single enzyme. This makes it a superior choice for cloning. Phusion DNA Polymerase produces blunt ends to the amplified fragment.
Long amplicons
Extension and overall cycling times can be significantly reduced when using Phusion DNA Polymerase. Due to the high processivity, Phusion DNA Polymerase can amplify long templates in a fraction of the time other polymerases need.
Contributors to this topic

JeffreyBarrick, CraigBarnhart, AurkoDasgupta, MichaelHammerling, MeghnaVergis
Topic revision: r1 - 2011-10-06 - 15:47:56 - Main.CraigBarnhart