Which polymerase is right for me?
Types of polymerase
Our lab stocks two main types of polymerase:
Type |
Vender |
Cost per unit |
Product Information |
Image |
Taq DNA polymerase |
Syzygy Biotech |
$0.18 |
link |

Standard Taq |
Phusion High Fidelity DNA polymerase |
NEB |
$0.86 |
link |

Phusion HF |
Applications
Phusion High Fidelity
High-fidelity PCR
The error rate of Phusion DNA Polymerase is 4.4 x 10^-7; therefore, Phusion DNA Polymerase is suitable for all PCR applications requiring great accuracy.
Cloning
Phusion DNA Polymerase amplifies templates with an accuracy and speed previously unattainable with a single enzyme. This makes it a superior choice for cloning. Phusion DNA Polymerase possesses 5' - 3' polymerase activity and 3'-5' exonuclease activity and will generate blunt ended products.
Long amplicons
Extension and overall cycling times can be significantly reduced when using Phusion DNA Polymerase. Due to the high processivity, Phusion DNA Polymerase can amplify long templates in a fraction of the time other polymerases need.
Contributors to this topic

JeffreyBarrick, CraigBarnhart, AurkoDasgupta, MeghnaVergis, MichaelHammerling
Topic revision: r3 - 2011-10-12 - 21:31:01 - Main.JeffreyBarrick
Lab.ProtocolsTaq moved from Lab.Taq on 2011-10-12 - 21:30 by Main.JeffreyBarrick -