(r3) Barrick Lab :: ProtocolsRecipesTetrazoliumSugar

---+++ TA: Tetrazolium Sugar (TA, TM, TL, ...)


Combine in a 2 L flask:
|  *1.5L*  |  *Component*  |
| 15 g | Tryptone | 
| 1.5 g | Yeast Extract |
| 7.5 g | NaCl | 
| 24 g | Agar | 
| 1.5 ml | 5% Antifoam | 
Add dH<sub>2</sub>O to 1.3 liter.

Combine in a 500 ml flask:
|  *1.5L*  |  *Component*  |
| 6 g | Sugar (arabinose = TA, maltose = TM, lactose = TL) | 
Add dH<sub>2</sub>O to 0.2 liter.

Autoclave sugar and media separately.
Total combined volume is 1.5 liters.

Combine and add:
|  *1.5L*  |  *Component*  |
| 1.5 ml | TTC (5%) | or | 7.5 ml | TTC (1%) |

Barrick Lab > ProtocolsMediaRecipes > ProtocolsRecipesTetrazoliumSugar

Contributors to this topic

JeffreyBarrick, CraigBarnhart, JordanMonk

Topic revision: r3 - 2011-07-29 - 14:43:44 - Main.CraigBarnhart