Sterile Saline

Purpose: Used make dilutions of viable cells for plating or transfer to new media.

This saline concentration of 0.85% w/v (145 mM) is suitable for diluting E. coli before transferring to new media or plating on agar.

1L 5L Component
8.5 g 42.5 g NaCl

Add dH2O to final volume. Autoclave before use. Prepare large batches in 6 L flask and then aliquot out into bottles. It is not important to measure out exact volumes when dividing a large batch among bottles.

Edit | Attach | Watch | Print version | History: r4 < r3 < r2 < r1 | Backlinks | Raw View | More topic actions

 Barrick Lab  >  ProtocolsRecipesSaline

Contributors to this topic Edit topic JeffreyBarrick, CraigBarnhart, AlvaroRodriguez
Topic revision: r4 - 2013-09-03 - 03:44:19 - Main.AlvaroRodriguez
 
This site is powered by the TWiki collaboration platform Powered by Perl This site is powered by the TWiki collaboration platformCopyright ©2024 Barrick Lab contributing authors. Ideas, requests, problems? Send feedback