Difference: ProtocolsRecipesSaline (1 vs. 4)

Revision 42013-09-03 - AlvaroRodriguez

 

Sterile Saline

Purpose: Used make dilutions of viable cells for plating or transfer to new media.

This saline concentration of 0.85% w/v (145 mM) is suitable for diluting E. coli before transferring to new media or plating on agar.

1L 5L Component
8.5 g 42.5 g NaCl
Changed:
<
<		Add H2O to final volume. Autoclave before use. Prepare large batches in 6 L flask and then aliquot out into bottles. It is not important to measure out exact volumes when dividing a large batch among bottles.
>
>		Add dH2O to final volume. Autoclave before use. Prepare large batches in 6 L flask and then aliquot out into bottles. It is not important to measure out exact volumes when dividing a large batch among bottles.
 

Revision 32011-07-29 - CraigBarnhart

 

Sterile Saline

Purpose: Used make dilutions of viable cells for plating or transfer to new media.

This saline concentration of 0.85% w/v (145 mM) is suitable for diluting E. coli before transferring to new media or plating on agar.

Changed:
<
<
1L 5L component
8.5 g 42.5 g NaCl
>
>
1L 5L Component
8.5 g 42.5 g NaCl
  Add H2O to final volume. Autoclave before use. Prepare large batches in 6 L flask and then aliquot out into bottles. It is not important to measure out exact volumes when dividing a large batch among bottles.

Revision 22011-05-19 - JeffreyBarrick

 

Sterile Saline

Added:
>
>		 Purpose: Used make dilutions of viable cells for plating or transfer to new media.
  This saline concentration of 0.85% w/v (145 mM) is suitable for diluting E. coli before transferring to new media or plating on agar.

1L 5L component
8.5 g 42.5 g NaCl

Add H2O to final volume. Autoclave before use. Prepare large batches in 6 L flask and then aliquot out into bottles. It is not important to measure out exact volumes when dividing a large batch among bottles.

Revision 12011-03-08 - JeffreyBarrick

 

Sterile Saline

This saline concentration of 0.85% w/v (145 mM) is suitable for diluting E. coli before transferring to new media or plating on agar.

1L 5L component
8.5 g 42.5 g NaCl

Add H2O to final volume. Autoclave before use. Prepare large batches in 6 L flask and then aliquot out into bottles. It is not important to measure out exact volumes when dividing a large batch among bottles.

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