(r6) Barrick Lab :: ProtocolsRecipesS2

S2

Used for Acinetobacter

1L	5L	Component
1.5g	7.5 g	KH₂PO₄
25.5g	127.5 g	Na₂HPO₄ 7H₂O
2g	10 g	NH₄Cl

After autoclaving, wait for containers to return to room temperature, then add the following sterile ingredients.

1L	Component
1ml	10% MgSO₄
0.09ul	1M CaCl₂
0.5ml	0.1% FeSO₄ 7H₂O
5 ml	90% Lactic Acid

The functional constraints are that Calcium Chloride cannot be autoclaved in the presence of sulfates, or it will precipitate as calcium sulfate. Ferrous sulfate when autoclaved will decompose to ferric sulfate, ferric oxide and sulfur dioxide. This means that either calcium chloride OR magnesium sulfate may be added before autoclaving, but not both.

Final composition
190.2 mM	Sodium (Na⁺)
11.0 mM	Potassium (K⁺)
37.8 mM	Ammonium (NH₄⁺)
0.09 mM	Calcium (Ca²⁺)
0.83 mM	Magnesium (Mg²⁺)
0.033 mM	Iron II (Fe²⁺)
38.0 mM	Chloride (Cl^-)
0.87 mM	Sulfate (SO₄^2-)
106.1 mM	Phosphate (PO₄^3-)
50.0 mM	Lactic Acid

-- Main.AurkoDasgupta - 03 Jun 2013

Barrick Lab > ProtocolsRecipesS2

Contributors to this topic

AurkoDasgupta, GabrielSuarez

Topic revision: r6 - 2013-06-27 - 15:18:45 - Main.AurkoDasgupta