(r2) Barrick Lab :: ProtocolsRecipesRCVMedium

---+++RCV Medium

RCV is a complex media consisting of 3 different sub-componets that must be prepared ahead of time if they are not already made.

*Main recipe*
|  *1L*  |  *Component*  |
| 10 mL | 10% (NH<sub>4</sub>)<sub>2</sub> SO<sub>4 |
| 40 mL | 10% DL-malate, pH 6.8 |
| 50 mL | Super salts (see below) |
| 15 mL | 0.64 KPO<sub>4</sub>, pH 6.8 (see below) |

add 3/4 H<sub>2</sub>O before adding the KPO<sub>4</sub>
adjust pH to 6.8 if needed
if making plates add 16 g Agar per L


 *0.64M KPO<sub>4</sub>, pH 6.8*
|  *500mL*  |  *Component*  |
| 20 g | KH<sub>2</sub>PO<sub>4 |
| 30 g | K<sub>2</sub>HPO<sub>4 |

*Super salts*
|  *1L*  |  *Component*  |
| 40 mL | 1.0% EDTA |
| 20 mL | 20% MgSO<sub>4</sub> 5H<sub>2</sub>O |
| 20 mL | 7.5% CaCl<sub>2</sub> 2H<sub>2</sub>O |
| 20 mL | Trace elements (see below) |
| 48 mL | 0.5% FeSO<sub>4</sub> 7H<sub>2</sub>O |
| 20 mL | 0.1% thiamine-HCl |

*Trace Elements*
|  *250mL*  |  *Component*  |
| 0.3975 g | MnSO<sub>4</sub>H<sub>2</sub>O |
| 0.7 g | H<sub>3</sub>BO<sub>3</sub> (Boric Acid) |
| 0.01 g | Cu(NO<sub>3</sub>)<sub>2</sub> 3H<sub>2</sub>O |
| 0.06 g | ZnSO<sub>4</sub> 7H<sub>2</sub>O |
| 0.1875 g | NaMoO<sub>4</sub> 2H<sub>2</sub>O (Sodium molybdate VI dihydrate) |

Barrick Lab > ProtocolsMediaRecipes > ProtocolsRecipesRCVMedium

Contributors to this topic

CraigBarnhart, JeffreyBarrick

Topic revision: r2 - 2012-08-01 - 16:04:50 - Main.CraigBarnhart