MG: Minimal Glucose, aka DM: Davis Minimal

Note: Salts, agar and sugars need to be prepared and autoclaved separately
Also note: When making these plates it is necessary to prepare the 3 main parts (salts, agar, and sugar) separately . Each must be prepared and autoclaved independent of the others. Please follow the order below.

1L 1.5L Component MW
7 g 10.5 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 3 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
333 ml 500 ml dH2O  
Autoclave.

Next, prepare the agar:

1L 1.5L Component
16 g 24 g Agar
1 ml 1.5 ml Antifoam (5%)
333 ml 500 ml dH2O
Autoclave.

Next, prepare the sugar (0.2%):

1L 1.5L Component
4 g 6 g Glucose (or other sugar)
333 ml 500 ml dH2O
Autoclave.

After the 3 parts have been autoclaved, combine the contents of the three flasks together and add the following stock solutions:

1L 1.5L Component
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 (autoclaved)
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) (filter sterilized)

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Contributors to this topic Edit topic JeffreyBarrick, CraigBarnhart, AlexaMorton, AurkoDasgupta
Topic revision: r4 - 2011-11-30 - 17:40:15 - Main.CraigBarnhart
 
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