(r1) Barrick Lab :: ProtocolsRecipesLysogenyBroth

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---+++ LB: Luria-Bertani

| *1L* | *5L* | *component* |
| 10 g | 50 g | Tryptone | 
| 5 g | 25 g | Yeast Extract |
| 10 g | 50 g | NaCl | 

Add dH<sub>2</sub>O to final volume. Autoclave. If mixing up large batches and aliquoting, be sure to add exact volumes to final media bottles so that they will be ready for addition of antibiotic to known concentrations.

*Note: This recipe is _different_ from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!*

Barrick Lab > ProtocolsRecipesLysogenyBroth

Contributors to this topic

JeffreyBarrick, SophiaLin, CraigBarnhart

Topic revision: r1 - 2011-03-08 - 14:19:03 - Main.JeffreyBarrick

Lab.ProtocolsRecipesLuriaBertani moved from Lab.ProtocolsRecipesSalineLuriaBertani on 2011-03-08 - 14:19 by Main.JeffreyBarrick