(r1) ProtocolsCulturingGilliamellaApicola < Lab

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---++ Working with _Gilliamella apicola_  

_Gilliamella apicola_ is a gram negative bacterial member of the honey bee (_Apis mellifera_) gut core microbiota that can be cultivated _in vitro_.  Its isolation and characterization were recently carried out by [[https://wkkwong.com][Waldan Kwong]] in the laboratory of [[http://web.biosci.utexas.edu/moran/][Dr. Nancy Moran]] and are described in depth in [[http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.044875-0][here]].

---+++ _In vitro_ culture conditions

---++++ Plate Growth
_Gilliamella apicola_ strain wkB1 (type strain) and wkB7 are generally cultured at 35°C and 5% CO<sub>2</sub>.  They grow robustly on agar plates after 48 hours on the following media:
   * Heart Infusion Agar
   * Heart Infusion Agar supplement with Sheep's Blood
 

---++++ Liquid Media
_Gilliamella apicola_ can be cultivated in the following liquid media (same general culture conditions as above, without shaking):
   * Insectagro (proprietary media)




-- Main.SeanLeonard - 25 Aug 2016
Topic revision: r1 - 2016-08-25 - SeanLeonard