(r1) Barrick Lab :: Protocols16SSequencing

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---+ 16S rRNA Sequencing to Identify Unknown Microbes

Ever wonder what that contaminant in your culture is? Need to accurately identify an environmental isolate?

---++ PCR Reaction

For PCR we use universal primers U341F and UA1406R that amplify an approximately 1 kb stretch of rDNA and should work for nearly any bacterial or archaeal sequence  [1].

| *primer* | *sequence* |
| U341F | CCTACGGGRSGCAGCAG |
| UA1406R | ACGGGCGGTGWGTRCAA |

---++ Analysis

Use the tools at the [[http://rdp.cme.msu.edu/][Ribosomal Protein Database]].

---++ References
1.	Baker, G.C., Smith, J.J. & Cowan, D.A. Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 55, 541-55 (2003).

Barrick Lab > ProtocolList > Protocols16SSequencing

Contributors to this topic

JeffreyBarrick, CraigBarnhart, ElizabethRobinson, ElizabethManriquez

Topic revision: r1 - 2010-05-13 - 16:29:25 - Main.JeffreyBarrick