topA Gene Sequencing
The topA gene coordinates are 1329420-1332017. The topA mutation in the Ara-1 long-term line is 1329516:C→T.| primer | who | coords | sequence |
|---|---|---|---|
| RL0398 | TC | 1329010-1329031 | 5'‑CGCGGTCGATGGGTTGTGTCTC |
| RL0399 | TC | 1332121-1332101 | 5'‑ATCATTTATAGCCTTAGTGTA |
| RL0400 | TC | 1329557-1329577 | 5'‑GAGTGCCGACTCTACCTCCAC |
| RL0401 | TC | 1330088-1330109 | 5'‑TGCCAGCACGACCACGCCATCT |
| RL0402 | TC | 1330609-1330629 | 5'‑AGTTCGTTGCCTGCCAGATGA |
| RL0403 | TC | 1331103-1331122 | 5'‑GCTGTACTGGATCACTTCTT |
| RL0410, RL0610 | RW, BW | 1329489-1329508 | 5'‑GACTACGTGGTGAAATCCAG |
| RL0411, RL0611 | RW, BW | 1329683-1329663 | 5'‑ACCAGGCAACACTTCATAGTG |
| RL0738 | CO | 1329010-1329029 | 5'‑CGCGGTCGATGGGTTGTGTC |
| RL0739 | CO | 1332323-1332302 | 5'‑TCCCGGGTTGTGATGTGTAAAG |
| RL0740 | CO | 1329648-1329670 | 5'‑CACAATTGGGAGGCGCACTATGA |
| RL0741 | CO | 1331694-1331672 | 5'‑GTAATGGCACCGGATCTTCTTTC |
| RL0742 | CO | 1329990-1330007 | 5'‑GCCTGTCTGCCGGTCGTGTG |
| RL0743 | CO | 1331055-1331032* | 5'‑TCTGCGCGGTAAAgTCGTAGTTCA |
Edit | Attach | Barrick Lab > ProtocolList > ProceduresTargetedSequencing > ProceduresSequencingTopA
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