ProceduresSequencingTopA < Lab

---+ _topA_ Gene Sequencing

The _topA_ gene coordinates are 1329420-1332017.

The _topA_ mutation in the Ara-1 long-term line is 1329516:C&rarr;T.

| *primer* | *who* | *coords* | *sequence* |
| RL0398 | TC | 1329010-1329031 | 5'&#8209;CGCGGTCGATGGGTTGTGTCTC |
| RL0399 | TC | 1332121-1332101 | 5'&#8209;ATCATTTATAGCCTTAGTGTA |
| RL0400 | TC | 1329557-1329577 | 5'&#8209;GAGTGCCGACTCTACCTCCAC |
| RL0401 | TC | 1330088-1330109 | 5'&#8209;TGCCAGCACGACCACGCCATCT |
| RL0402 | TC | 1330609-1330629 | 5'&#8209;AGTTCGTTGCCTGCCAGATGA |
| RL0403 | TC | 1331103-1331122 | 5'&#8209;GCTGTACTGGATCACTTCTT |
| RL0410, RL0610 | RW, BW | 1329489-1329508 | 5'&#8209;GACTACGTGGTGAAATCCAG |
| RL0411, RL0611 | RW, BW | 1329683-1329663 | 5'&#8209;ACCAGGCAACACTTCATAGTG |
| RL0738 | CO | 1329010-1329029 | 5'&#8209;CGCGGTCGATGGGTTGTGTC |
| RL0739 | CO | 1332323-1332302 | 5'&#8209;TCCCGGGTTGTGATGTGTAAAG |
| RL0740 | CO | 1329648-1329670 | 5'&#8209;CACAATTGGGAGGCGCACTATGA |
| RL0741 | CO | 1331694-1331672 | 5'&#8209;GTAATGGCACCGGATCTTCTTTC |
| RL0742 | CO | 1329990-1330007 | 5'&#8209;GCCTGTCTGCCGGTCGTGTG |
| RL0743 | CO | 1331055-1331032* | 5'&#8209;TCTGCGCGGTAAAgTCGTAGTTCA |

* This primer has a base mismatch (lowercase) to REL606. It must have been designed based on the MG1655 sequence.

All coordinates are for the reference strain REL606.

Barrick Lab > ProtocolList > ProceduresTargetedSequencing > ProceduresSequencingTopA

Topic revision: r1 - 2009-08-05 - 14:51:18 - Main.JeffreyBarrick