ProceduresASKAEvolutionExperiment < Lab

---+ ASKA Collection Evolution Experiment

---++ Starting Lines

   1 Revive each strain by inoculating a scrape of frozen culture from a well of a microplate into 3 ml of 0.1x LB + 30 &mu;g/ml chloramphenicol. Grow overnight.
   1 Precondition to the growth medium by transferring 11.7 &mu;l of each culture into 3 ml of fresh 0.1x LB + 30 &mu;g/ml chloramphenicol. Grow overnight.
   1 Found evolutionary independent replicates of each strain by initiating one culture _for each experimental replicate_ from a small number (100-1000) of cells. If you are founding three strain mixtures, you need three copies of each strain that will be present in each mixture at this step. Grow for <font color="red">2 days</font> to ensure that each strain reaches a similar saturating density from the small inoculum.
   1 Now begin the evolution experiment. Mix together equal volumes of each culture. Transfer from this mixture the daily dilution into fresh media and grow that culture overnight.

Barrick Lab > ProtocolList > ProceduresASKAEvolutionExperiment

Topic revision: r1 - 2009-09-23 - 21:15:51 - Main.JeffreyBarrick