---+ ASKA Collection Evolution Experiment ---++ Starting Lines 1 Revive each strain by inoculating a scrape of frozen culture from a well of a microplate into 3 ml of 0.1x LB + 30 μg/ml chloramphenicol. Grow overnight. 1 Precondition to the growth medium by transferring 11.7 μl of each culture into 3 ml of fresh 0.1x LB + 30 μg/ml chloramphenicol. Grow overnight. 1 Found evolutionary independent replicates of each strain by initiating one culture _for each experimental replicate_ from a small number (100-1000) of cells. If you are founding three strain mixtures, you need three copies of each strain that will be present in each mixture at this step. Grow for <font color="red">2 days</font> to ensure that each strain reaches a similar saturating density from the small inoculum. 1 Now begin the evolution experiment. Mix together equal volumes of each culture. Transfer from this mixture the daily dilution into fresh media and grow that culture overnight.
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Topic revision: r1 - 2009-09-23 - JeffreyBarrick