(r1) Barrick Lab :: ExperimentalqPCR

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---+ Experimental qPCR Plate Setup and Analysis

_Goals_

   * Test hypothesis
   * Typical plate setup with 2 reference genes and a target gene:

<table width="355" cellspacing="0" style="width: 355pt; margin-left: 4.65pt; border-collapse: collapse" cellpadding="0" border="0"> <tbody><tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> </td> <td width="40" valign="bottom" style="width: 40pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *1* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *2* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *3* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *4* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *5* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *6* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *7* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *8* </p> </td> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt"> <p align="right" style="text-align: right"> *9* </p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt">


*A*
</td> <td width="47" valign="bottom" style="width: 46.75pt; border: 1pt solid windowtext; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"><p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR1</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt">

*B*
</td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR2</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt">

*C*
</td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR3</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt">

*D*
</td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR4</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt">

*E*
</td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C1BR5</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt">

*F*
</td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR1</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt">

*G*
</td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR2</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt">

*H*
</td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR3</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt">

*I*
</td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR4</p> </td> </tr> <tr style="height: 15pt"> <td width="35" valign="bottom" style="width: 35pt; padding: 0in 5.4pt; height: 15pt">

*J*
</td> <td width="40" valign="bottom" style="width: 40pt; border-width: medium 1pt 1pt; border-style: none solid solid; border-image-source: none; border-image-slice: initial; border-image-width: initial; border-image-outset: initial; border-image-repeat: initial; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #e4dfec; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #dce6f1; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> <td width="35" valign="bottom" style="width: 35pt; border-width: medium 1pt 1pt medium; border-style: none solid solid none; background: none 0% 0% repeat scroll #fcd5b4; padding: 0in 5.4pt; height: 15pt"> <p align="center" style="text-align: center">C2BR5</p> </td> </tr> </tbody></table>

_Conditions_

   * If you want to be extra safe you should also include a water control for each primer pair.
   * The cDNA dilution used for the experimental samples will be those determined from the cDNA Concentration qPCR, and you should use at least your best 2 reference genes from the Reference Gene qPCR

_What you are looking for_

   * Technical replicates with a standard deviation below 0.2.
   * Outliers; if you have an SD for a technical triplicate higher than 0.2, and there is obviously an outlier, remove it. An example is if you have values of 23.34, 23.35 and 30.22, and the 30.22 amplification trace is clearly poor. If the SD is higher than 0.2 and all three measurements are spread, you must keep all three.

    

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Barrick Lab > ProtocolList > QPCR > ExperimentalqPCR
Contributors to this topic
KateElston, JuliePerreau
Topic revision: r1 - 2019-03-21 - 22:01:15 - Main.KateElston