(r3) Barrick Lab :: ProtocolsTaq

---+ Which polymerase is right for me?

---++ Types of polymerase
Our lab stocks two main types of polymerase:  <br>
| *Type* | *Vender* | *Cost per unit* | *Product Information* | *Image* |
| Taq DNA polymerase | Syzygy Biotech | $0.18 | [[https://www.syzygybiotech.com/Home/Products][link]] | <img style="border-color: black;  border-style: solid; border-width: 1px;" width=180 height=120 src="%ATTACHURL%/Taq.jpg"><br><div align=center style="font-size:10pt">Standard Taq</div> |
| Phusion High Fidelity DNA polymerase | NEB | $0.86 | [[http://www.neb.com/nebecomm/tech_reference/polymerases/phusion_high.asp][link]] | <img style="border-color: black;  border-style: solid; border-width: 1px;" width=180 height=120  src="%ATTACHURL%/Phusion.jpg"><br><div align=center style="font-size:10pt">Phusion HF </div> |

---++ Applications

*Phusion High Fidelity* <br>

_High-fidelity PCR_ <br>
The error rate of Phusion DNA Polymerase is 4.4 x 10^-7; therefore, Phusion DNA Polymerase is suitable for all PCR applications requiring great accuracy.

_Cloning_ <br>
Phusion DNA Polymerase amplifies templates with an accuracy and speed previously unattainable with a single enzyme. This makes it a superior choice for cloning. Phusion DNA Polymerase possesses 5' - 3' polymerase activity and 3'-5' exonuclease activity and will generate blunt ended products.

_Long amplicons_ <br>
Extension and overall cycling times can be significantly reduced when using Phusion DNA Polymerase. Due to the high processivity, Phusion DNA Polymerase can amplify long templates in a fraction of the time other polymerases need.

Barrick Lab > ProtocolList > ProtocolsTaq

Contributors to this topic

CraigBarnhart, AurkoDasgupta, JeffreyBarrick, MeghnaVergis, MichaelHammerling

Topic revision: r3 - 2011-10-12 - 21:31:01 - Main.JeffreyBarrick

Lab.ProtocolsTaq moved from Lab.Taq on 2011-10-12 - 21:30 by Main.JeffreyBarrick -