(r3) ProtocolsRecipesS2 < Lab

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Used for Acinetobacter

|  *1L*  |  *5L*  |  *Component*  |
| 1.5g | 7.5 g | KH<sub>2</sub>PO<sub>4</sub>| 
| 25.5g | 127.5 g | Na<sub>2</sub>HPO<sub>4</sub> 7H<sub>2</sub>O| 
| 0.1g | 0.5 g | MgSO<sub>4</sub>| 
| 2g | 10 g | NH<sub>4</sub>Cl| 
| 0.01g | 0.05 g | CaCl<sub>2</sub>| 
| 0.0005g | 0.0025 g | FeSO<sub>4</sub> 7H<sub>2</sub>O| 


To each 1L, add 5ml of 85-90% lactic acid. Bring final pH to 6.65.

Calcium Chloride may be better added as 1ml of 1% solution, or 90ul of 1M solution.

Ferrous Sulfate may be better added as 0.5ml of a 0.1% solution.

| *Final composition* ||
| 95.1 mM | Sodium (Na<sup>+</sup>) |
| 11.0 mM | Potassium (K<sup>+</sup>) |
| 37.8 mM | Ammonium (NH<sub>4</sub>) | 
| 0.09 mM | Calcium (Ca<sup>2+</sup>) |
| 0.83 mM | Magnesium (Mg<sup>2+</sup>) |
| 38.0 mM | Chloride (Cl<sup>-</sup>|
| 0.87 mM | Sulfate (SO<sub>4</sub><sup>2-</sup>) |
| 106.1 mM | Phosphate (PO<sub>4</sub><sup>3-</sup>) |
| 50.0 mM | Lactic Acid|

-- Main.AurkoDasgupta - 03 Jun 2013

Barrick Lab > ProtocolsRecipesS2

Topic revision: r3 - 2013-06-21 - 20:59:47 - Main.AurkoDasgupta