LB: Luria-Bertani

1L 5L component
10 g 50 g Tryptone
5 g 25 g Yeast Extract
10 g 50 g NaCl

Add dH2O to final volume. Autoclave. If mixing up large batches and aliquoting, be sure to add exact volumes to final media bottles so that they will be ready for addition of antibiotic to known concentrations.

Note: This recipe is different from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!

Edit | Attach | Watch | Print version | History: r6 | r4 < r3 < r2 < r1 | Backlinks | Raw View | More topic actions...

 Barrick Lab  >  ProtocolsRecipesLysogenyBroth

Contributors to this topic Edit topic JeffreyBarrick, CraigBarnhart
Topic revision: r1 - 2011-03-08 - 14:19:03 - Main.JeffreyBarrick
Lab.ProtocolsRecipesLuriaBertani moved from Lab.ProtocolsRecipesSalineLuriaBertani on 2011-03-08 - 14:19 by Main.JeffreyBarrick
 
This site is powered by the TWiki collaboration platform Powered by Perl This site is powered by the TWiki collaboration platformCopyright ©2024 Barrick Lab contributing authors. Ideas, requests, problems? Send feedback