PA: Lac Papillation Agar

Make 10× Minimal A Salts.

After autoclaving, combine agar and sugar and add:

1L	Component
1 ml	500 mg/ml P-Gal (phenyl β-D-galactoside) (filter sterilized)
1 ml	40 mg/ml X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactoside) (filter sterilized)
2.5 ml	0.2% w/v Thiamin (vitamin B1) (filter sterilized)
6 ml	10% w/v (0.083M Mg2+) Magnesium Sulfate, anhydrous MgSO4 (autoclaved) MW 120.37
100 ml	10× Minimal A Salts

Note: The amounts added of some standard components (including agar) are slightly different for this recipe. Volumes have also been altered from the original Miller recipe to use the same stock solutions we have available for making DM plates. The final concentrations are 0.2% glucose, 0.05% P-Gal, and 0.004% X-Gal (w/v).

Sources:

1. Nghiem, Y., Cabrera, M., Cupples, C.G., Miller, J.H. (1988) The mutY gene : A mutator locus in Escherichia coli that generates G•C→T•A transversions. Proc. Natl. Acad. Sci. U.S.A. 85:2709-2713.
2. Miller J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.