This is start of a protocol to split pooled samples which have internal barcodes into individual fastq files.

  1. copy pooled files from corral to scratch
  2. gunzip files
  3. generate a 2 column tsv with sample name and combined R1R2 barcode with no spaces
  4. run the dual_inline_bc_assignment.py script from barricklab repository
  5. copy new .fastq files back to corral

-- Main.DanielDeatherage - 31 May 2016

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Contributors to this topic Edit topic DanielDeatherage
Topic revision: r1 - 2016-05-31 - 19:54:51 - Main.DanielDeatherage
 
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