ProtocolsFindStrainsPlasmids < Lab

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---+ Find Strains, Plasmids, and Genes
 Reminder: Always revive new organisms according to an established protocol and archive a lab stock of the original freeze-dried culture after it has been resuspended, in addition to the initial culture, and cultures from picked colonies.

---++ General culture collections
   * [[https://www.atcc.org/][American Type Culture Collection (ATCC)]]
      * Note: ATCC is usually much more expensive than other sources.
      * See the [[https://www.atcc.org/en/Products/Cells_and_Microorganisms/Bacteria/Preceptrol_Cultures.aspx][ATCC Bacterial Preceptrol Cultures]] for the most economical cultures.
   * [[https://www.dsmz.de/catalogues/catalogue-microorganisms.html][German Collection of Microorganisms and Cell Cultures (DSMZ)]]
   * [[http://www.ncimb.com/][UK National Collection of Industrial Food and Marine Bacteria (NCIMB)]]
   * [[http://nrrl.ncaur.usda.gov/][USDA Agricultural Research Service Culture Collection (NRLL)]]
   * [[http://www.wfcc.info/ccinfo/home/][Culture Collections Information Worldwide (WDCM)]] %BR% This is a database of culture collections from all over the world.

---++ Strain collections for specific species
   * [[http://cgsc.biology.yale.edu/][E. coli Genetic Stock Center]]

---++ Plasmid collections
   * [[https://www.addgene.org/][Addgene]] %BR%Most common source for published plasmids.
   * [[http://parts.igem.org/Main_Page][Registry of Standard Biological Parts]]%BR%We have the [[http://parts.igem.org/assembly/libraries.cgi][iGEM distributions]] from 2012 and 2014&ndash;present.
   * [[http://seva.cnb.csic.es][Standard European Vector Architecture 2.0 (SEVA)]]%BR%Similar to iGEM repository, but uses a different standard that allows backbone elements (like the origin) to vary.

---++ Advice for choosing strains
   * Whenever possible, choose a strain that has a *complete genome sequence* available. You can check by searching on the [[https://www.ncbi.nlm.nih.gov/genome/][NCBI Genome Page]]. After you do a search, be sure to click on the link by "Organism Overview ; Genome Assembly and Annotation report [XXXX]" to see a table of all XXXX of the genomes with sequencing data. Ideally, you want ones with a level of "complete".
Barrick Lab > ProtocolList > ProtocolsFindStrainsPlasmids
Topic revision: r4 - 2018-06-05 - 16:26:54 - Main.JeffreyBarrick