(r1) ProtocolsCulturingFirm

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---++ Working with Lactobacillus _"Firm-5"_
 <h3 style="padding-right: 6px; padding-left: 0.5em; margin-top: -4px; margin-right: -13px; margin-bottom: 15px; font-weight: 500; font-size: 18.154499053955078px; border-top-style: none; border-right-style: none; border-left-style: none; background-color: #ffffff; color: #000000; border-bottom-color: black; font-family: 'Palatino Linotype', 'Book Antiqua', Palatino, serif; clear: both; background-image: none; position: relative; width: auto">

The <em>Lactobacillus "Firm 5" </em>clade are gram positive bacterial symbionts of the honey bee ( _Apis mellifera_) gut core microbiota that can be cultivated _in vitro_. Their isolation and characterization were recently carried out by <a target="_top" href="https://wkkwong.com/" style="text-decoration: none; color: #666666; border-color: #58438b; border-style: none none dotted; border-width: 1px">Waldan Kwong</a> in the laboratory of <a target="_top" href="http://web.biosci.utexas.edu/moran/" style="text-decoration: none; color: #666666; border-color: #58438b; border-style: none none dotted; border-width: 1px">Dr. Nancy Moran</a> and are described in depth <a target="_top" href="http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.044875-0" style="text-decoration: none; color: #666666; border-color: #58438b; border-style: none none dotted; border-width: 1px">here</a>. Genomes for two Firm-5 strains, wkB8 and wkB10 are described [[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4241670/][here]]

_In vitro_ culture conditions
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---++++ <a name="Plate_Growth"></a>Plate Growth
 _Firm 5_ strains wkB8 and wkB10 are generally cultured at 35°C and 5% CO<sub style="font-family: 'Palatino Linotype', 'Book Antiqua', Palatino, serif">2</sub>. They grow robustly on agar plates after 48 hours on the following media: 
   * Columbia Agar supplement with Sheep's Blood
   * MRS agar

---++++ <a name="Liquid_Media"></a>Liquid Media
 _Firm 5_ strains wkB8 and wkB10 can be cultivated in the following liquid media (same general culture conditions as above, without shaking): 
   * MRS broth

-- Main.SeanLeonard - 23 Feb 2017
Topic revision: r1 - 2017-02-23 - SeanLeonard