ProtocolsCulturingArsenophonusNasoniae < Lab

---+ Working with _Arsenophonus nasoniae_

_Arsenophonus nasoniae_ is symbiont of the wasp, <em>Nasonia vitripennis, </em>gut microbiota that can be cultivated _in vitro_. The isolation and characterization of the particular strain (15247) used was carried out by Dr. Robert L. Gherna and are described in depth in [[http://www.microbiologyresearch.org/docserver/fulltext/ijsem/41/4/ijs-41-4-563.pdf?expires=1487884673&id=id&accname=sgid026149&checksum=3A244793D35672397E881B8D6CE2EA26][here]].

A second <em>A. nasoniae </em>strain (24899) was isolated from the tick, <em>Ixodes ricinus, </em>by Oleg Mediannikov et. al and is described [[http://www.sciencedirect.com/science/article/pii/S1877959X1200101X][here]].

---+++ _In vitro_ culture conditions

---++++ Plate Growth
 _Arsenophonus nasoniae_ DSMZ Strain: [[https://www.dsmz.de/catalogues/details/culture/DSM-15247.html?tx_dsmzresources_pi5[returnPid]=304][15247]] can be cultured at room temperature; although recommended culturing temperature found in literature is 28°C. It grows robustly on agar plates after 3-5 days on the following media: 
   * Brain Heart Infusion Agar

This particular strain doesn't show any antibiotic resistance to antibiotics at E. coli concentrations, so all standard lab concentrations can be used when making plates.

<em>Arsenophonus nasoniae</em> DSMZ Strain: [[https://www.dsmz.de/catalogues/details/culture/DSM-24899.html][24899]] can be cultured at room temperature; although recommended culturing temperature found in literature is 28°C. It grows robustly on agar plates after &gt;1 week on the following media:
   * Colombia Blood Agar

---++++ Liquid Media

<em>Arsenophonus nasoniae </em>15247 can be cultivated in the following liquid media (same general culture conditions as above, with shaking):

   * Brain Heart Infusion Media

Strain 24899 can be cultivated in the following liquid media (same general culture conditions as above, with shaking):

   * Brain Heart Infusion Media
   * Trypticase Soy Broth

-- Main.KateElston - 23 Feb 2017
Barrick Lab > ProtocolList > ProtocolsCulturingArsenophonusNasoniae
Topic revision: r2 - 2017-05-25 - 19:36:44 - Main.KateElston