(r1) Barrick Lab :: ProceduresPulsedFieldGelElectrophoresisMapping

---+ Pulsed Field Gel Electrophoresis Mapping

Purpose: To validate mutations predicted from whole-genome re-sequencing and possibly discover rearrangements through repetitive sequences or tandem duplications that are difficult to predict from sequencing data.

---++ Preparation of Plugs

---++ Digestion with Restriction Enzyme

---++ Running Pulsed Field Gel

---++ Analysis of Gel