(r3) Barrick Lab :: ProceduresChangingEnvironmentLongTerm

---+++ 

Contamination check. Set up plates with a checkerboard pattern of Ara+ and Ara- marked cells. On odd-numbered plates an Ara- is in well A1.  On even-numbered plates an Ara+ strain is in well A1.

Daily, transfer 3.5 µl into 900 µl of fresh DM0 + 0.01% carbon compound.

At regular intervals, take a plate after it has

---++++ Plate 1

Red Plate. Strains 606/607. 8 Replicates, one per column.
| 1 | 1 | D-Glucose |
| 2 | 2 | Glycerol |
| 3 | 3 | &beta;-Lactose |
| 4 | 4 | D-Fructose |
| 5 | 5 | D-Ribose |
| 6 | 6 | D-Galactose |
| 7 | 7 | L-Rhamnose |
| 8 | 8 | Melibiose |
| 9 | 9 | D-Gluconic Acid |
| 10 | 10 | D-Sorbitol |
| 11 | 11 | Acetate |
| 12 | 12 | L-Malic Acid |

---++++ Plate 2

White Plate. Strains 606/607. 8 Replicates, one per column.
| 1 | 13 | Pyruvic Acid |
| 2 | 14 | DL-Lactic Acid |
| 3 | 17 | Thymidine |
| 4 | 18 | Inosine |
| 5 | 19 | D-Trehalose|
| 6 | 20 | D-Serine |
| 7 | 21 | D-Glucuronic Acid |
| 8 | 22 | L-Alanine |
| 9 | 23 | D-Glucosamine |
| 10 | 37 | Glucuronamide |
| 11 | 71 | D-Arabinose |
| 12 | 72 | L-Proline |

---++++ Plate 3

Red Plate. Strains 606/607. 8 Replicates, one per column.
|  | Treatment | Days |
| 1 | L-Proline, Thymidine, Acetate, D-Glucose | 1 |
| 2 | L-Proline, Thymidine, Acetate, D-Glucose | 2 |
| 3 | L-Proline, Thymidine, Acetate, D-Glucose | 4 |
| 4 | L-Proline, Thymidine, Acetate, D-Glucose | 8 |
| 5 | L-Proline, Thymidine, Acetate, D-Glucose | 16 |
| 6 | L-Proline, Thymidine, Acetate, D-Glucose | 32 |
| 7 | Pyruvic Acid, D-Galactose, &beta;-Lactose, D-Glucose | 1 |
| 8 | Pyruvic Acid, D-Galactose, &beta;-Lactose, D-Glucose | 2 |
| 9 | Pyruvic Acid, D-Galactose, &beta;-Lactose, D-Glucose | 4 |
| 10 | Pyruvic Acid, D-Galactose, &beta;-Lactose, D-Glucose | 8 |
| 11 | Pyruvic Acid, D-Galactose, &beta;-Lactose, D-Glucose | 16 |
| 12 | Pyruvic Acid, D-Galactose, &beta;-Lactose, D-Glucose | 32 |

---++++ Plate 4

White Plate. Strains 606/607. 8 Replicates, one per column.
|  | Treatment | Days |
| 1 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 1 |
| 2 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 2 |
| 3 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 4 |
| 4 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 8 |
| 5 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 16 |
| 6 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 32 |
| 7 | Acetate, D-Ribose, Glycerol, D-Glucose | 1 |
| 8 | Acetate, D-Ribose, Glycerol, D-Glucose | 2 |
| 9 | Acetate, D-Ribose, Glycerol, D-Glucose | 4 |
| 10 | Acetate, D-Ribose, Glycerol, D-Glucose | 8 |
| 11 | Acetate, D-Ribose, Glycerol, D-Glucose | 16 |
| 12 | Acetate, D-Ribose, Glycerol, D-Glucose | 32 |

---++++ Plate 5

Red Plate. Strains 606/607. 8 Replicates, one per column.
|  | Treatment | Days |
| 1 | Random 1 | 1 |
| 2 | Random 2 | 1 |
| 3 | Random 3 | 1 |
| 4 | Random 4 | 1 |
| 5 | Random 5 | 1 |
| 6 | Random 6 | 1 |
| 7 | Random 7 | 1 |
| 8 | Random 8 | 1 |
| 9 | Random 9 | 1 |
| 10 | Random 10 | 1 |
| 11 | Random 11 | 1 |
| 12 | Random 12 | 1 |

-- Main.JeffreyBarrick - 24 Sep 2007
Barrick Lab > ProceduresChangingEnvironmentLongTerm
Contributors to this topic
JeffreyBarrick, MarkKauth
Topic revision: r3 - 2007-09-24 - 17:17:39 - Main.JeffreyBarrick