(r1) Barrick Lab :: GoldenGateAssemblyProtocolsMainPage

-- Main.DennisMishler - 14 Dec 2017

This page serves as the main repository for everything Golden Gate Assembly-related. This page links to a number of protocol pages that will help you design DNA sequences for Golden Gate Assembly, learn about the various different Golden Gate Assembly techniques and when we employ each one, and conduct the different types of Golden Gate Assembly reactions.

Golden Gate Assembly (GGA) was first described in [[http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0003647][Engler C, Kandzia R, Marillonnet S (2008)]] and %BLUE%<u>[[http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0005553][Engler C, Gruetzner R, Kandzia R, Marillonnet S (2009)]]</u>%ENDCOLOR% as an efficient way to quickly assembly multiple DNA sequences, or parts, into a single plasmid.

List of Golden Gate Assembly subpages

   * <span style="background-color: transparent; text-indent: -0.25in">[[ProtocolsGoldenGateAssembly][Yeast Toolkit (YTK) - Derived from Lee, Dueber 2015]]</span>
   * <span style="background-color: transparent; text-indent: -0.25in">[[BroadHostRangeToolkit][Broad Host range Toolkit (BTK) - Derived from Leonard, Barrick 2018]]</span>
   * <span style="background-color: transparent; text-indent: -0.25in">[[ProtocolsBTKDesignANewPart][Designing a new DNA part sequence]]</span>
   * <span style="background-color: transparent; text-indent: -0.25in">[[ProtocolsBTKGoldenGateAssemblyofPartPlasmids][Creating a part plasmid using BsmBI]]</span>
   * [[ProtocolsGoldenGateAssemblyOfCassettePlasmids][Creating a cassette plasmid using BsaI]]

Work in Progress. As of 12/14/2017, none of the above links are guaranteed to work or be accurate. This is a work in progress reorganization of the Barrick lab Golden Gate Assembly Protocols, including those used by the Microbe Hackers undergraduate research lab and UT Austin iGEM team.

-Dr. Mishler

Barrick Lab > ProtocolList > GoldenGateAssemblyProtocolsMainPage

Contributors to this topic

KateElston, DennisMishler, SeanLeonard, AlexaMorton, JeffreyBarrick, JuliePerreau

Topic revision: r1 - 2017-12-14 - 22:02:09 - Main.DennisMishler