(r3) Barrick Lab :: EvolvabilityLongTermPlates

---++++ Plate 1: 12 single carbon sources

General design considerations:
   1 Begin growth in substrate that supports lower cell density.

Red Plate. Strains 606/607. 8 Replicates, one per column.
| 1 | 1 | D-Glucose | 1 |
| 2 | 2 | D-Mannose | 1? |
| 3 | 3 | D-Galactose | 1 |
| 4 | 4 | Glycerol | 1 |
| 5 | 5 | D-Ribose | 2 |
| 6 | 6 | &beta;-Lactose  | 2 |
| 7 | 7 | L-Rhamnose | 4 |
| 8 | 8 | L-Alanine | 4 |
| 9 | 9 | Pyruvate | 4 |
| 10 | 10 | D-Sorbitol | 5 |
| 11 | 11 | Acetate | 8 |
| 12 | 12 | L-Malic Acid | 8 |

---++++ Plate 2:  glucose / lactose scan + 5 carbon source pairs
| 1 | 6/1 | &beta;-Lactose / D-Glucose, 1 transfer  |
| 2 | 6/1 | &beta;-Lactose / D-Glucose, 2 transfers |
| 3 | 6/1 | &beta;-Lactose / D-Glucose, 4 transfers |
| 4 | 6/1 | &beta;-Lactose / D-Glucose, 8 transfers |
| 5 | 6/1 | &beta;-Lactose / D-Glucose, 16 transfers |
| 6 | 6/1 | &beta;-Lactose / D-Glucose, 32 transfers |
| 7 | 6/1 | &beta;-Lactose / D-Glucose, 64 transfers |
| 8 | 2/1 | D-Mannose / D-Glucose, 8 transfers |
| 9 | 3/1 | D-Galactose / D-Glucose |
| 10 | 4/1 | Glycerol / D-Glucose |
| 11 | 5/1 | D-Ribose / D-Glucose |
| 12 | 7/1 | L-Rhamnose / D-Glucose | 

---++++ Plate 3:  glucose / pyruvate scan + 5 carbon source pairs
| 1 | 9/1 | Pyruvate / D-Glucose, 1 transfer  |
| 2 | 9/1 | Pyruvate / D-Glucose, 2 transfers |
| 3 | 9/1 | Pyruvate / D-Glucose, 4 transfers |
| 4 | 9/1 | Pyruvate / D-Glucose, 8 transfers |
| 5 | 9/1 | Pyruvate / D-Glucose, 16 transfers |
| 6 | 9/1 | Pyruvate / D-Glucose, 32 transfers |
| 7 | 9/1 | Pyruvate / D-Glucose, 64 transfers |
| 8 | 8/1 | L-Alanine / D-Glucose, 8 transfers |
| 9 | 10/1 | D-Sorbitol / D-Glucose, 8 transfers |
| 10 | 11/1 | Acetate / D-Glucose, 8 transfers |
| 11 |  | |
| 12 |  | |

2-4
2-5
2-6
2-7
2-10
2-11
3-5
3-6
3-7
3-9
3-10
4-7
4-8
4-9
4-10
5-6
5-10
5-11
5-12
6-8
6-12
7-8
7-9
7-10
7-12
8-11
8-12
9-10
10-11
10-12
11-12

---++++ Plate 4:  glucose / L-malate scan + 5 carbon source pairs
| 1 | 12/1 | D-Glucose / L-Malate, 1 transfer  |
| 2 | 12/1 | D-Glucose / L-Malate, 2 transfers |
| 3 | 12/1 | D-Glucose / L-Malate, 4 transfers |
| 4 | 12/1 | D-Glucose / L-Malate, 8 transfers |
| 5 | 12/1 | D-Glucose / L-Malate, 16 transfers |
| 6 | 12/1 | D-Glucose / L-Malate, 32 transfers |
| 7 | 12/1 | D-Glucose / L-Malate, 64 transfers |

---++++ Plate 5:  12 carbon source pairs

---++++ Plate 6:  12 carbon source pairs


---+++ First Generation Plates
---++++ Plate 1

Red Plate. Strains 606/607. 8 Replicates, one per column.
| 1 | 1 | D-Glucose |
| 2 | 2 | Glycerol |
| 3 | 3 | &beta;-Lactose |
| 4 | 4 | D-Fructose |
| 5 | 5 | D-Ribose |
| 6 | 6 | D-Galactose |
| 7 | 7 | L-Rhamnose |
| 8 | 8 | Melibiose |
| 9 | 9 | D-Gluconic Acid |
| 10 | 10 | D-Sorbitol |
| 11 | 11 | Acetate |
| 12 | 12 | L-Malic Acid |

---++++ Plate 2

White Plate. Strains 606/607. 8 Replicates, one per column.
| 1 | 13 | Pyruvic Acid |
| 2 | 14 | DL-Lactic Acid |
| 3 | 17 | Thymidine |
| 4 | 18 | Inosine |
| 5 | 19 | D-Trehalose|
| 6 | 20 | D-Serine |
| 7 | 21 | D-Glucuronic Acid |
| 8 | 22 | L-Alanine |
| 9 | 23 | D-Glucosamine |
| 10 | 37 | Glucuronamide |
| 11 | 71 | D-Arabinose |
| 12 | 72 | L-Proline |

---++++ Plate 3

Red Plate. Strains 606/607. 8 Replicates, one per column.
|  | Treatment | Transfers |
| 1 | L-Proline, Thymidine, Acetate, D-Glucose | 1 |
| 2 | L-Proline, Thymidine, Acetate, D-Glucose | 2 |
| 3 | L-Proline, Thymidine, Acetate, D-Glucose | 4 |
| 4 | L-Proline, Thymidine, Acetate, D-Glucose | 8 |
| 5 | L-Proline, Thymidine, Acetate, D-Glucose | 16 |
| 6 | L-Proline, Thymidine, Acetate, D-Glucose | 32 |
| 7 | Pyruvic Acid, D-Galactose, &beta;-Lactose, D-Glucose | 1 |
| 8 | Pyruvic Acid, D-Galactose, &beta;-Lactose, D-Glucose | 2 |
| 9 | Pyruvic Acid, D-Galactose, &beta;-Lactose, D-Glucose | 4 |
| 10 | Pyruvic Acid, D-Galactose, &beta;-Lactose, D-Glucose | 8 |
| 11 | Pyruvic Acid, D-Galactose, &beta;-Lactose, D-Glucose | 16 |
| 12 | Pyruvic Acid, D-Galactose, &beta;-Lactose, D-Glucose | 32 |

---++++ Plate 4

White Plate. Strains 606/607. 8 Replicates, one per column.
|  | Treatment | Transfers |
| 1 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 1 |
| 2 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 2 |
| 3 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 4 |
| 4 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 8 |
| 5 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 16 |
| 6 | L-Rhamnose, D-Galactose, D-Fructose, D-Glucose | 32 |
| 7 | Acetate, D-Ribose, Glycerol, D-Glucose | 1 |
| 8 | Acetate, D-Ribose, Glycerol, D-Glucose | 2 |
| 9 | Acetate, D-Ribose, Glycerol, D-Glucose | 4 |
| 10 | Acetate, D-Ribose, Glycerol, D-Glucose | 8 |
| 11 | Acetate, D-Ribose, Glycerol, D-Glucose | 16 |
| 12 | Acetate, D-Ribose, Glycerol, D-Glucose | 32 |
Barrick Lab > ProtocolList > EvolvabilityLongTermPlates
Contributors to this topic
JeffreyBarrick
Topic revision: r3 - 2007-11-10 - 15:56:29 - Main.JeffreyBarrick