Commonly Used Plasmids

plasmid markers origin ref description
pCA24N CamR     ASKA collection vector
pKD46 AmpR     Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3 CamR     Wanner template plasmid
pKD13 KanR     Wanner template plasmid
pCR2.1 KanR, AmpR     TOPO cloning kit vector
pACYC184 TetR, CamR     Lenski evolution experiment
pKO3, pKOV SucS, CamR     Church vectors for creating deletions
pRA300, pRA301, pRA302 SpecR   Akakura2002 Broad host range, promoterless lacZ map

References

[Akakura2002] Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780.

Attachments Attachments
Topic attachments
I Attachment History Action Size Date Who Comment
Unknown file formatgbk pACYC184.gbk r1 manage 7.7 K 2009-08-03 - 21:09 JeffreyBarrick  
Unknown file formatgbk pCA24N.gbk r1 manage 9.9 K 2009-05-29 - 13:21 JeffreyBarrick  
Unknown file formatgbk pCR2.1.gbk r2 r1 manage 7.1 K 2012-03-07 - 21:11 JeffreyBarrick  
Unknown file formatgbk pKD13_Kan_template.gbk r1 manage 7.6 K 2009-05-29 - 13:22 JeffreyBarrick  
Unknown file formatgbk pKD3_Cam_template.gbk r1 manage 6.6 K 2009-05-29 - 13:22 JeffreyBarrick  
Unknown file formatgb pKD46.gb r1 manage 16.4 K 2012-01-20 - 21:09 BrianRenda lambda red plasmid genbank file
Texttxt pKOV.txt r1 manage 10.6 K 2011-02-21 - 17:13 JeffreyBarrick  
Unknown file formatgbk pkd4.gbk r1 manage 6.1 K 2011-12-19 - 22:29 BrianRenda  
Unknown file formatgbk pkd4_with_gfp_sites_marked.gbk r1 manage 10.0 K 2011-12-19 - 22:35 BrianRenda  
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Topic revision: r13 - 2012-03-28 - JeffreyBarrick