Predicted mutation
evidence seq id position mutation annotation gene description
MC JC REL606 3,289,962 Δ16 bp coding (96‑111/4554 nt) gltB glutamate synthase, large subunit

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 3289962 3289977 16 49 [0] [0] 49 gltB glutamate synthase, large subunit

New junction evidence
  seq id position reads (cov) reads (cov) score skew annotation gene product
* ? REL606 = 32899610 (0.000)48 (0.880) 34/70 0.2 coding (95/4554 nt) gltB glutamate synthase, large subunit
?REL606 3289978 = 0 (0.000)coding (112/4554 nt) gltB glutamate synthase, large subunit

TGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCC  >  REL606/3289943‑3290013
                                   |                                   
gggttcccgcagagcctgggggaggttcacgatatc                                     <  1:4136029‑M2/1‑1 (MQ=255)
gggttcccgcagagcctgagggaggttcacgatatc                                     >  1:5136776‑M2/36‑36 (MQ=255)
  gttcccgcagagcctgggggaggttcacgatatCtt                                   >  1:3327311‑M2/34‑36 (MQ=255)
     cccgcagagcctgggggaggttcacgatatCTTgag                                <  1:321637‑M2/6‑1 (MQ=255)
      ccgcagagcctgggggaggttcacgatatCTTgaga                               <  1:1435538‑M2/7‑1 (MQ=255)
      ccgcagagcctgggggaggttcacgatatCTTgaga                               >  1:728798‑M2/30‑36 (MQ=255)
      ccgcagagcctgggggaggttcacgatatCTTgaga                               <  1:2435189‑M2/7‑1 (MQ=255)
      ccgcagagcctgggggaggttcacgatatCTTgaga                               >  1:2519051‑M2/30‑36 (MQ=255)
       cgcagagcctgggggaggttcacgatatCTTGagag                              >  1:5330195‑M2/29‑36 (MQ=255)
       cgcagagcctgggggaggttcacgatatCTTGagag                              >  1:4473088‑M2/29‑36 (MQ=255)
        gcagagcctgggggaggttcacgatatCTTGAGAgg                             >  1:2113304‑M2/28‑36 (MQ=255)
        gcagagcctgggggaggttcacgatatCTTGAGAgg                             >  1:4912198‑M2/28‑36 (MQ=255)
         cagagcctgggggaggttcacgatatCTTGAGAggg                            >  1:7224075‑M2/27‑36 (MQ=255)
         cagagcctgggggaggttcacgatatCTTGAGAggg                            >  1:5472267‑M2/27‑36 (MQ=255)
            agcctgggggaggttcacgatatCTTGAGAGGGATa                         >  1:7100640‑M2/24‑36 (MQ=255)
              cctgggggaggttcacgatatCTTGAGAGGTATAAc                       >  1:2719396‑M2/22‑36 (MQ=255)
              cctgggggaggttcacgatatCTTGAGAGGGATAAc                       <  1:4509504‑M2/15‑1 (MQ=255)
              cctgggggaggttcacgatatCTTGAGAGGGATAAc                       <  1:2617914‑M2/15‑1 (MQ=255)
               ctggggggggttcacgatatCTTGAGAGGGATAACt                      <  1:6565020‑M2/16‑1 (MQ=255)
               ctgggggaggttcacgatatCTTGAGAGGGTTAACt                      >  1:3928093‑M2/21‑36 (MQ=255)
               ctgggggaggttcacgatatCTTGAGAGGGATAACt                      >  1:3070297‑M2/21‑36 (MQ=255)
                 gggggaggttcacgatatCTTGAGAGGGATAACtgt                    >  1:925597‑M2/19‑36 (MQ=255)
                 gggggaggttcacgatatCTTGAGAGGGATAACtgt                    >  1:1583709‑M2/19‑36 (MQ=255)
                 gggggaggttcacgatatCTTGAGAGGGATAACtgt                    <  1:1678757‑M2/18‑1 (MQ=255)
                  ggggaggttcacgatatCTTGAGAGGGATAACtgtg                   >  1:3231936‑M2/18‑36 (MQ=255)
                   gggaggttcacgatatCTTGAGAGGGATAACTGTgg                  >  1:7267416‑M2/17‑36 (MQ=255)
                   gggaggttcacgatatCTTGAGAGGGATAACTGTgg                  <  1:4806166‑M2/20‑1 (MQ=255)
                    ggaggttcacgatatCTTGAGAGGGATAACTGTGGt                 <  1:500979‑M2/21‑1 (MQ=255)
                    ggaggttcacgatatCTTGAGAGGGATAACTGTGGt                 <  1:6348773‑M2/21‑1 (MQ=255)
                     gaggttcacgatatCTTGAGAGGGATAACTGTGGtt                <  1:3537557‑M2/22‑1 (MQ=255)
                     gaggttcacgatatCTTGAGAGGGATAACTGTGGtt                >  1:4975305‑M2/15‑36 (MQ=255)
                     gaggttcacgatatCTTGAGAGGGATAACTGTGGtt                <  1:6287136‑M2/22‑1 (MQ=255)
                     gagattcacgatatCTTGAGAGGGATAACTGTGGtt                <  1:4049854‑M2/22‑1 (MQ=255)
                      aggttcacgatatCTTGAGAGGGATAACTGTGGttt               >  1:280225‑M2/14‑36 (MQ=255)
                      aggttcacgatatCTTGAGAGGGATAACTGTGGttt               <  1:2623833‑M2/23‑1 (MQ=255)
                      aggttcacgatatCTTGAGAGGGATAACTGTGGttt               <  1:5674725‑M2/23‑1 (MQ=255)
                       ggttcacgatatCTTGAGAGGGATAACTGTGGTTTc              <  1:4707617‑M2/24‑1 (MQ=255)
                        gttcacgatatCTTGAGAGGGATAACTGTGGTTTCg             <  1:1818014‑M2/25‑1 (MQ=255)
                        gttcacgatatCTTGAGAGGGATAACTGTGGTTTCg             >  1:3566879‑M2/12‑36 (MQ=255)
                          tcacgatatCTTGAGAGGGATAACTGTGGTTTCGGc           <  1:6843675‑M2/27‑1 (MQ=255)
                           cacgatatCTTGAGAGGGATAACTGTGGTTTTGGcc          >  1:2533717‑M2/9‑36 (MQ=255)
                             cgatatCTTGAGAGGGATAACTGTGGTTTCGGCctt        >  1:2887498‑M2/7‑35 (MQ=255)
                             cgatatCTTGAGAGGGATAACTGTGGTTTCGGCCTg        >  1:5849235‑M2/7‑36 (MQ=255)
                             cgatatCTTGAGAGGGATAACTGTGGTTTCGGCCTg        <  1:7070671‑M2/30‑1 (MQ=255)
                             cgatatCTTGAGAGGGATAACTGTGGTTTCGGCCTg        >  1:1604243‑M2/7‑36 (MQ=255)
                                 atCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCg    <  1:3025852‑M2/34‑1 (MQ=255)
                                  tCTTGAGAGGGATAACTGTGGTTTCGGCCTGTTCGc   >  1:2174694‑M2/2‑36 (MQ=255)
                                  tCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGc   <  1:7461580‑M2/35‑1 (MQ=255)
                                   cTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGcc  <  1:2605912/36‑1 (MQ=255)
                                   |                                   
TGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCC  >  REL606/3289943‑3290013

Base quality scores:  ATCG  < 3 ≤  ATCG  < 11 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 32 ≤  ATCG