BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	REL606	3,482,398	Δ25 bp	100%	coding (714‑738/2706 nt)	malT →	transcriptional regulator MalT

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	3482398	3482422	25	147 [0]	[0] 149	malT	transcriptional regulator MalT

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 3482397	0 (0.000)	108 (1.140)	56/80	NT	100%	coding (713/2706 nt)	malT	transcriptional regulator MalT
*	?	REL606	3482423 =	0 (0.000)	108 (1.140)	56/80	NT	100%	coding (739/2706 nt)	malT	transcriptional regulator MalT

Only 100 of 149 total aligned reads displayed.

GGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATAACGTC  >  REL606/3482373‑3482473

ctacagctaatcgccctctccgcccggcagaatacccactcagcccataaa                                                    >  1:3711632‑M2/51‑51 (MQ=255)

ctacagctaatcgccctctccgcccggcagaatacccactcagcccataaa                                                    <  1:6715266‑M2/1‑1 (MQ=255)

ctacagctaatcgccctctccgcccggcagaatacccactcagcccataaa                                                    >  1:9679710‑M2/51‑51 (MQ=255)

ctacagctaatcgccctctccgcccggcagaatacccactcagcccataaa                                                    <  1:8924177‑M2/1‑1 (MQ=255)

ctacagctaatcgccctctccgcccggcagaatacccactcagcccataaa                                                    <  1:4074017‑M2/1‑1 (MQ=255)

 tacagctaatcgccctctccgcccggcagaatacccactcagcccataaaa                                                   <  1:3545570‑M2/2‑1 (MQ=255)

 tacagctaatcgccctctccgcccggcagaatacccactcagcccataaaa                                                   >  1:5574620‑M2/50‑51 (MQ=255)

 tacagctaatcgccctctccgcccggcagaatacccactcagcccataaaa                                                   <  1:9749478‑M2/2‑1 (MQ=255)

  acagctaatcgccctctccgcccggcagaatacccactcagcccataaAAt                                                  <  1:3090495‑M2/3‑1 (MQ=255)

  acagctaatcgccctctccgcccggcagaatacccactcagcccataaAAt                                                  <  1:9077641‑M2/3‑1 (MQ=255)

  acagctaatcgccctctccgcccggcagaatacccactcagcccataaAAt                                                  <  1:6197904‑M2/3‑1 (MQ=255)

  acagctaatcgccctctccgcccggcagaatacccactcagcccataaAAt                                                  >  1:3089801‑M2/49‑51 (MQ=255)

    agctaatcgccctctccgcccggcagaatacccactcagcccataaAATgc                                                <  1:9349548‑M2/5‑1 (MQ=255)

     gctaatcgccctctccgcccggcagaatacccactcagcccataaAATgcc                                               <  1:9919367‑M2/6‑1 (MQ=255)

      ctaattgccctctccgcccggcagaatacccactcagcccataaAATgcca                                              >  1:4659355‑M2/45‑51 (MQ=255)

      ctaatcgccctctccgcccggcagaatacccactcagcccataaAATgcca                                              >  1:10175745‑M2/45‑51 (MQ=255)

      ctaatcgccctctccgcccggcagaatacccactcagcccataaAATgcca                                              <  1:5461121‑M2/7‑1 (MQ=255)

        aatcgccctctccgcccggcagaatacccactcagcccataaAATgccagc                                            <  1:10235424‑M2/9‑1 (MQ=255)

        aatcgccctctccgcccggcagaatacccactcagcccataaAATgccagc                                            <  1:1688997‑M2/9‑1 (MQ=255)

          tcgccctctccgcccggcagaatacccactcagcccataaAATgccagcca                                          <  1:3203014‑M2/11‑1 (MQ=255)

           cgccctctccgcccggcagaatacccactcagcccataaAATGCCAGCCAt                                         <  1:4817858‑M2/12‑1 (MQ=255)

           cgccctctccgcccggcagaatacccactcagcccataaAATGCCAGCCAt                                         >  1:1204110‑M2/40‑51 (MQ=255)

           cgccctctccgcccggcagaatacccactcagcccataaAATGCCAGCCAt                                         <  1:5423310‑M2/12‑1 (MQ=255)

            gccctctccgcccggcagaatacccactcagcccataaAATGCCAGCCATc                                        >  1:4023598‑M2/39‑51 (MQ=255)

              cctctccgcccggcagaatacccactcagcccataaAATGCCAGCCATCtt                                      <  1:2022854‑M2/15‑1 (MQ=255)

              cctctccgcccggcagaatacccactcagcccataaAATGCCAGCCATCtt                                      <  1:127474‑M2/15‑1 (MQ=255)

               ctctccgcccggcagaatacccactcagcccataaAATGCCAGCCATCttt                                     <  1:3911900‑M2/16‑1 (MQ=255)

               ctctccgcccggcagaatacccactcagcccataaAATGCCAGCCATCttt                                     <  1:1008253‑M2/16‑1 (MQ=255)

               ctctccgcccggcagaatacccactcagcccataaAATGCCAGCCATCttt                                     <  1:1352796‑M2/16‑1 (MQ=255)

                tctccgcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTc                                    >  1:10016745‑M2/35‑51 (MQ=255)

                tctccgcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTc                                    <  1:7334941‑M2/17‑1 (MQ=255)

                tctccgcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTc                                    <  1:8603797‑M2/17‑1 (MQ=255)

                 ctccgcccggcagaataccccctcagcccataaAATGCCAGCCATCTTTCg                                   <  1:7813115‑M2/18‑1 (MQ=255)

                 ctccgcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCg                                   <  1:5536470‑M2/18‑1 (MQ=255)

                 ctccgcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCg                                   <  1:7623992‑M2/18‑1 (MQ=255)

                 ctccgcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCg                                   <  1:9627594‑M2/18‑1 (MQ=255)

                  tccgcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCgg                                  <  1:5179512‑M2/19‑1 (MQ=255)

                  tccgcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCgg                                  >  1:4300491‑M2/33‑51 (MQ=255)

                  tccgcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCgg                                  <  1:79259‑M2/19‑1 (MQ=255)

                  tccgcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCgg                                  >  1:9297572‑M2/33‑51 (MQ=255)

                  tccgcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCgg                                  >  1:6798550‑M2/33‑51 (MQ=255)

                    cgcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGAt                                >  1:1685605‑M2/31‑51 (MQ=255)

                    cgcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGAt                                <  1:8654496‑M2/21‑1 (MQ=255)

                    cgcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGAt                                <  1:10414945‑M2/21‑1 (MQ=255)

                     gcccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGAtt                               <  1:10875020‑M2/22‑1 (MQ=255)

                      cccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTa                              >  1:9253684‑M2/29‑51 (MQ=255)

                      cccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTa                              <  1:7108401‑M2/23‑1 (MQ=255)

                      cccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTa                              <  1:10397090‑M2/23‑1 (MQ=255)

                       ccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTAt                             <  1:9739837‑M2/24‑1 (MQ=255)

                       ccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTAt                             >  1:9764287‑M2/28‑51 (MQ=255)

                       ccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTAt                             <  1:7881938‑M2/24‑1 (MQ=255)

                       ccggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTAt                             >  1:3392860‑M2/28‑51 (MQ=255)

                        cggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTATc                            >  1:1898042‑M2/27‑51 (MQ=255)

                        cggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTATc                            >  1:10066128‑M2/27‑51 (MQ=255)

                         ggcagaataccctctcagcccataaAATGCCAGCCATCTTTCGGATTATCt                           <  1:9148227‑M2/26‑1 (MQ=255)

                         ggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTATCt                           <  1:8441919‑M2/26‑1 (MQ=255)

                         ggcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTATCt                           <  1:2279443‑M2/26‑1 (MQ=255)

                          gcagaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTg                          >  1:4706819‑M2/25‑51 (MQ=255)

                           cagaatacccactcagcccataaAATGCCAGCTATCTTTCGGATTATCTgg                         >  1:2610674‑M2/24‑51 (MQ=255)

                            agaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGt                        <  1:9506223‑M2/29‑1 (MQ=255)

                            agaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGt                        >  1:5653263‑M2/23‑51 (MQ=255)

                             gaatacccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTc                       <  1:9715142‑M2/30‑1 (MQ=255)

                              aatacccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCg                      <  1:8535751‑M2/31‑1 (MQ=255)

                               atacccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGa                     <  1:6281906‑M2/32‑1 (MQ=255)

                                tacccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGAt                    >  1:10708308‑M2/19‑51 (MQ=255)

                                tacccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGAt                    <  1:7761779‑M2/33‑1 (MQ=255)

                                tacccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGAt                    <  1:6589388‑M2/33‑1 (MQ=255)

                                 acccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATg                   >  1:7628864‑M2/18‑51 (MQ=255)

                                 acccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATg                   >  1:2617190‑M2/18‑51 (MQ=255)

                                  cccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATNa                  <  1:1983335‑M2/35‑1 (MQ=255)

                                   ccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAg                 <  1:6946101‑M2/36‑1 (MQ=255)

                                   ccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAg                 >  1:4857404‑M2/16‑51 (MQ=255)

                                   ccactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAg                 <  1:10491075‑M2/36‑1 (MQ=255)

                                    cactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAgg                <  1:5673844‑M2/37‑1 (MQ=255)

                                    cactcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAgg                >  1:7504889‑M2/15‑51 (MQ=255)

                                     actcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGt               <  1:8642450‑M2/38‑1 (MQ=255)

                                      ctcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAg                 <  1:4470921‑M2/36‑1 (MQ=255)

                                      ctcagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGtt              <  1:9493466‑M2/39‑1 (MQ=255)

                                        cagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGtttt            <  1:536316‑M2/41‑1 (MQ=255)

                                        cagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGtttt            <  1:10032484‑M2/41‑1 (MQ=255)

                                        cagcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGtttt            <  1:9319358‑M2/41‑1 (MQ=255)

                                         agcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTg           >  1:10471970‑M2/10‑51 (MQ=255)

                                          gcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTgg          >  1:8658271‑M2/9‑51 (MQ=255)

                                          gcccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTgg          <  1:2435073‑M2/43‑1 (MQ=255)

                                           cccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGa         <  1:3255170‑M2/44‑1 (MQ=255)

                                            ccataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGAt        >  1:137687‑M2/7‑51 (MQ=255)

                                             cataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATa       >  1:10940071‑M2/6‑51 (MQ=255)

                                             cataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATa       <  1:3491434‑M2/46‑1 (MQ=255)

                                              ataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATaa      >  1:6741041‑M2/5‑51 (MQ=255)

                                              ataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATaa      <  1:6548572‑M2/47‑1 (MQ=255)

                                              ataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATaa      >  1:3660536‑M2/5‑51 (MQ=255)

                                              ataaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATaa      <  1:4151773‑M2/47‑1 (MQ=255)

                                                aaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATAACg    >  1:3398469‑M2/3‑51 (MQ=255)

                                                aaAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATAACg    <  1:9890450‑M2/49‑1 (MQ=255)

                                                 aAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATAACGt   >  1:7444656‑M2/2‑51 (MQ=255)

                                                 aAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATAACGt   <  1:5472493‑M2/50‑1 (MQ=255)

                                                 aAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATAACGt   >  1:1072688‑M2/2‑51 (MQ=255)

                                                 aAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATAACGt   <  1:10113367‑M2/50‑1 (MQ=255)

                                                  aaTGCCGGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATAACGTc  >  1:8912077/1‑51 (MQ=255)

                                                  aaTGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATAACGTc  <  1:1882140/51‑1 (MQ=255)

GGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATAACGTC  >  REL606/3482373‑3482473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`