Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	929,574	IS150 (+) +3 bp	coding (946‑948/1653 nt)	hcp ←	hydroxylamine reductase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 666130	NA (NA)	60 (0.950)	51/196	0.2	96.7%	noncoding (1443/1443 nt)	IS150	repeat region
	?	REL606	929574 =	2 (0.030)					coding (948/1653 nt)	hcp	hydroxylamine reductase
*	?	REL606	= 929576	2 (0.030)	71 (1.100)	52/200	0.2	97.2%	coding (946/1653 nt)	hcp	hydroxylamine reductase
	?	REL606	= 2775877	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

AATCATCACCGTCCAGATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/929477‑929576 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCAC  <  REL606/2775877‑2775780                                                                                                                                                                                                        AATCATCACCGTCCAGATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTT                                                                                                   >  2:1363988/1‑101 AATCATCACCGTCCAGATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTT                                                                                                   >  2:868222/1‑101     cTCACCGTCCAGATGACCCACTCCAGGCCAGCCAACAATGCTGCCGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTAC                                                                                               <  2:1451436/100‑1     ATCACCGTCCAGATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTct                                                                                               <  1:1202982/101‑3     ATCACCGTCCAGATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTAC                                                                                               <  1:1462725/101‑1     ATCACCGTCCAGATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTAC                                                                                               <  2:432260/101‑1     ATCACCGTCCAGATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTAC                                                                                               <  2:641858/101‑1      TCACCGTCCAGATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACT                                                                                              <  2:1336256/101‑1           GTCCAGATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACC                                                                                         >  1:917874/1‑101           GTCCAGATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACC                                                                                         >  1:1620063/1‑101               AGATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATT                                                                                     <  1:403542/101‑1               AGATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATT                                                                                     >  1:1184325/1‑101                GATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTT                                                                                    <  1:501052/101‑1                 ATGACGCACGCCAGTCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCCCCCAGTTT                                                                                   <  2:1613648/101‑1                 ATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTT                                                                                   <  2:1309465/101‑1                 ATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTT                                                                                   >  1:1614884/1‑101                    ACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTT                                                                                >  2:964163/1‑101                      GCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGG                                                                              <  1:1558984/101‑1                        ACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGAC                                                                            <  1:1495039/101‑1                           CCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGAT                                                                         <  2:1299812/101‑1                             cGGCCACCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGA                                                                       <  2:825540/100‑1                              GGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAA                                                                      <  1:1679543/101‑1                                CCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAAT                                                                    <  2:189833/101‑1                                 CAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATG                                                                   <  1:868227/101‑1                                 CAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATG                                                                   >  1:399800/1‑101                                    CCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAA                                                                >  1:100964/1‑101                                    CCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAA                                                                <  1:1293656/101‑1                                     CAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAAT                                                               >  2:956175/1‑101                                       ACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCCCCCATTTTGTTGGACGATGAAATGGAATAG                                                             <  1:261765/101‑1                                        CAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGC                                                            <  1:1409559/101‑1                                        CAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGC                                                            >  2:542194/1‑101                                               GCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                     <  1:1616171/101‑1                                                CGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATA                                                    >  2:384593/1‑101                                                CGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATA                                                    >  1:488927/1‑101                                                 GGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                   <  1:1522012/101‑1                                                  GGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATG                                                  >  1:1341866/1‑101                                                   GTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGT                                                 <  2:1621415/101‑1                                                     CCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCA                                               >  2:716053/1‑101                                                       AGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAA                                             >  2:825498/1‑101                                                         ATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGC                                           <  2:1184320/101‑1                                                         ATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGC                                           <  1:883853/101‑1                                                           ACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCA                                         >  1:216619/1‑101                                                                CGTCATAAGGGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATA                                    <  2:1202599/101‑1                                                                 GTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAC                                   <  1:1316009/101‑1                                                                  TCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACC                                  <  1:1431893/101‑1                                                                  TCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACC                                  >  1:743331/1‑101                                                                  TCATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACC                                  >  1:1688585/1‑101                                                                    ATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCT                                >  1:186604/1‑101                                                                    ATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCT                                >  2:499834/1‑101                                                                    ATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCT                                >  1:861799/1‑101                                                                    ATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCT                                >  1:1373531/1‑101                                                                    ATAAGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCT                                >  1:930019/1‑101                                                                       AGCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTT                             <  1:542199/101‑1                                                                        GCGCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTG                            <  1:246648/101‑1                                                                          GCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA                          <  2:1341860/101‑1                                                                          GCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA                          >  2:921088/1‑101                                                                          GCCTACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA                          >  2:72259/1‑101                                                                              ACGGTTGGGTCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGC                      >  2:834362/1‑101                                                                                       TCGATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAG             <  2:917869/101‑1                                                                                         GATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTC           >  1:1495395/1‑101                                                                                         GATGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTC           >  1:1213009/1‑101                                                                                           TGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGT         >  2:370327/1‑101                                                                                           TGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGT         >  1:787029/1‑101                                                                                           TGATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGT         >  1:585294/1‑101                                                                                             ATGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGA       >  1:1454564/1‑101                                                                                              TGCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAA      <  2:399795/101‑1                                                                                               GCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAAT     <  2:1614878/101‑1                                                                                               GCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAAT     >  1:1536648/1‑101                                                                                               GCAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAAT     <  2:930014/101‑1                                                                                                CAGTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATC    <  2:1373525/101‑1                                                                                                  GTTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCAC  <  2:787024/101‑1                                                                                                                                                                                                        AATCATCACCGTCCAGATGACGCACGCCAGGCCAGCCAACAATGCTGCGGGTCCAGATACGATCGTCATAAGCGCCTACGGTTGGGTCGATGATGCAGTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/929477‑929576 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCAC  <  REL606/2775877‑2775780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.