Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	929,574	IS150 (+) +3 bp	coding (946‑948/1653 nt)	hcp ←	hydroxylamine reductase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 666130	NA (NA)	60 (0.950)	51/196	0.2	96.7%	noncoding (1443/1443 nt)	IS150	repeat region
	?	REL606	929574 =	2 (0.030)					coding (948/1653 nt)	hcp	hydroxylamine reductase
*	?	REL606	= 929576	2 (0.030)	71 (1.100)	52/200	0.2	97.2%	coding (946/1653 nt)	hcp	hydroxylamine reductase
	?	REL606	= 2775877	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

AAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/666032‑666130 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACG  >  REL606/929574‑929672                                                                                                                                                                                                        AAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGT                                                                                                   >  1:330738/1‑101  AGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTT                                                                                                  <  2:811721/101‑1    AATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCG                                                                                                >  1:384031/1‑101       TAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGG                                                                                             >  2:1045266/1‑101           CTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCAT                                                                                         <  1:1279005/101‑1           CTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCAT                                                                                         >  1:481750/1‑101             GAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCA                                                                                       <  1:1179236/101‑1                 TTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGAT                                                                                   <  2:1478361/101‑1                    AAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGG                                                                                <  1:920126/101‑1                    AAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGG                                                                                <  1:217360/101‑1                    AAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGG                                                                                >  1:1328237/1‑101                        GTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCA                                                                            <  1:1111356/101‑1                         TCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAG                                                                           <  1:53880/101‑1                          CTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGG                                                                          >  2:838526/1‑101                          CTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGG                                                                          >  1:561332/1‑101                            ggCTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGA                                                                        <  2:123607/99‑1                             ACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAA                                                                       >  1:1454521/1‑101                              CTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAA                                                                      >  1:1401889/1‑101                              CTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAA                                                                      <  1:449720/101‑1                               TCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAAC                                                                     <  2:1269921/101‑1                                  AATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAG                                                                  <  1:679286/101‑1                                  AATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAG                                                                  <  2:1574149/101‑1                                  AATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAG                                                                  >  2:864368/1‑101                                   ATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGC                                                                 <  1:864373/101‑1                                      GAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAA                                                              >  2:1118445/1‑101                                       AATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAAC                                                             <  2:481745/101‑1                                        ATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACT                                                            >  1:1530411/1‑101                                            CGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCAC                                                        >  1:1684668/1‑101                                                ATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGC                                                    >  1:807760/1‑101                                                  CAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTG                                                  <  1:1360456/101‑1                                                   AGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGA                                                 >  2:116684/1‑101                                                      CCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTC                                              <  2:873511/101‑1                                                      CCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTC                                              <  1:710481/101‑1                                                       CTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCT                                             >  2:1098708/1‑101                                                       CTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCT                                             >  2:1604428/1‑101                                                       CTATATGCCTCGCGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCT                                             <  1:1300716/101‑1                                                          TATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCC                                          <  2:337903/101‑1                                                          TATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCC                                          >  1:1625753/1‑101                                                           ATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAGCTCCACTTGCTGATTCTGCCA                                         <  2:1410324/101‑1                                                           ATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCA                                         <  1:1022978/101‑1                                                              CCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCC                                      <  2:1308037/101‑1                                                               CTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCG                                     >  1:1455794/1‑101                                                                 CGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCT                                   <  2:1333553/101‑1                                                                 CGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCT                                   <  1:1128862/101‑1                                                                  GTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTG                                  >  1:125912/1‑101                                                                    GTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCC                                >  1:825734/1‑101                                                                    GTTTAACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCC                                >  1:1093273/1‑101                                                                        AACTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAG                            <  1:1705506/101‑1                                                                          CTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTT                          <  1:1445383/101‑1                                                                          CTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTT                          <  2:1271559/101‑1                                                                          CTGTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTT                          >  2:333395/1‑101                                                                            GTCCAACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTAC                        >  1:1155423/1‑101                                                                                 ACTTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACC                   <  2:199664/101‑1                                                                                   TTTTTGGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGATCTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAG                 <  1:374254/101‑1                                                                                        GGGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCT            <  2:1648737/101‑1                                                                                         GGGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTT           >  1:770962/1‑101                                                                                          GGTCAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTG          <  1:1102783/101‑1                                                                                             CAGTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAAT       <  2:1093268/101‑1                                                                                               GTACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTT     >  1:1557425/1‑101                                                                                                ttCAGATCGAGTTCATCACGATGGGGCCAGGGAACCGAGCGAACTCCACTTTCTGATTCTGCCCGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTA    <  1:592807/99‑1                                                                                                TACAGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTA    <  2:825729/101‑1                                                                                                  CAGTTCGAGGTCATCACGATGGGGCCAGCGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACG  <  2:1408244/101‑1                                                                                                                                                                                                        AAGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/666032‑666130 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caGTTCGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACG  >  REL606/929574‑929672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.