Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC REL606 3,841,192 IS150 (+) +3 bp 100% coding (389‑391/1074 nt) recF ← recombination protein F

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 3841192 =0 (0.000)115 (1.210) 58/80 NT 100% coding (391/1074 nt) recF recombination protein F
?REL606 = 3894996 NA (NA)noncoding (1443/1443 nt) IS150 repeat region
* ? REL606 = 38411940 (0.000)119 (1.320) 60/76 NT 100% coding (389/1074 nt) recF recombination protein F
?REL606 3893554 = NA (NA)noncoding (1/1443 nt) IS150 repeat region

GACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGAC  >  REL606/3894949‑3894999
                                               |   
gACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGAc  <  1:1768627/51‑1 (MQ=35)
                                               |   
GACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGAC  >  REL606/3894949‑3894999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.