Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	REL606	2,196,656	IS150 (–) +3 bp	100%	coding (1350‑1352/1992 nt)	cirA ←	ferric iron‑catecholate outer membrane transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 666130	NA (NA)	28 (1.060)	21/276	NT	100%	noncoding (1443/1443 nt)	IS150	repeat region
	?	REL606	= 2196658	0 (0.000)					coding (1350/1992 nt)	cirA	ferric iron‑catecholate outer membrane transporter
*	?	REL606	2196656 =	0 (0.000)	20 (0.750)	13/278	NT	100%	coding (1352/1992 nt)	cirA	ferric iron‑catecholate outer membrane transporter
	?	REL606	3651091 =	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

CGCCACCGACACCGTAACGGTGAAAGGGGGCTGGGCGACGGCATTTAAAGCACCTTCTCTGTTGCAACTTAGCCCTGACTGGACGAGCAATTCCTGCCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2196799‑2196656 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACG  >  REL606/3651091‑3651229                                                                                                                                                                                                                                                                                             CGCCACCGACACCGTAACGGTGAAAGGGGGCTGGGCGACGGCATTTAAAGCACCTTCTCTGTTGCAACTTAGCCCTGACTGGACGAGCAATTCCTGCCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACa                                                                                                                                       >  1:452534/1‑149                    GTGAAAGGGGGCTGGGCGACGGCATTTAAAGCACCTTCTCTGTTGCAACTTAGCCCTGACTGGACGAGCAATTCCTGCCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACG                                                                                                                    >  1:212534/1‑150                             GGCTGGGCGACGGCATTTAAAGCACCTTCTCTGTTGCAACTTAGCCCTGACTGGACGAGCAATTCCTGCCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGG                                                                                                           <  2:237085/150‑1                                 GGGCGCCGGCATTTAAAGCACCTTCTCAGTTGCAACTTAGCCCTGACTGGACGAGCAATGCCTGCAGTGGCGCAGGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATA                                                                                                       >  1:604063/1‑150                                     GACGGCATTTAAAGCACCTTCTCTGTTGCAACTTAGCCCTGACTGGACGAGCAATTCCTGCCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCC                                                                                                   <  2:626069/150‑1                                          CATTTAAAGCACCTTCTCTGTTGCAACTTAGCCCTGACTGGACGAGCAATTCCTGCCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                                                              <  1:479664/150‑1                                          CATTTAAAGCACCTTCTCTGTTGCAACTTAGCCCTGACTGGACGAGCAATTCCTGCCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                                                              <  2:453817/150‑1                                          CATTTAAAGCACCTTCTCTGTTGCAACTTAGCCCTGACTGGACGAGCAATTCCTGCCGTGGCCCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATTAAATGGAATAGCCCCTAAT                                                                                              <  1:233581/150‑1                                           ATTTAAAGCACCTTCTCTGTTGCAACTTAGCCCTGACTGGACGAGCAATTCCTGCCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATA                                                                                             >  1:426682/1‑150                                                           CTGTTGCAACTTAGCCCTGACTGGACGAGCAATTCCTGCCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATA                                                                             >  2:9898/1‑150                                                           CTGTTGCAACTTAGCCCTGACTGGACGAGCAATTCCTACCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATA                                                                             >  1:365622/1‑150                                                                   ACTTAGCCCTGACTGGACGAGCAATTCCTGCCGTGGGGTATGTATGATGGGGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCCCCCATTTTGTTGGACGATGAAATGCAATAGCCCCTATAATGTCAAAGCTAAAATGCCCTTTTG                                                                     >  1:209048/1‑150                                                                   ACTTAGCCCTGACTGGACGAGCAATTCCTGCCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTG                                                                     >  2:392261/1‑150                                                                      TAGCCCTGACTGGACGAGCAATTCCTGCCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAA                                                                  <  2:366681/150‑1                                                                                   ACGAGCAATTCCTGCCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGT                                                     <  2:213145/150‑1                                                                                                atgtgtataagagacAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTAC                                        <  2:510746/135‑1                                                                                                GCCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCATAATATGTCAAAGACAAACTACCCTTTTGAAAAGCGCATTGACGTCGTGACTCACTAC                                        >  1:63900/1‑150                                                                                                        tgtataagagacagGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAAC                                <  1:339822/136‑1                                                                                                          ATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTG                              >  2:569606/1‑150                                                                                                                      GGGTAGCCCGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACctgtctcttataca                  >  2:340798/1‑136                                                                                                                              CGGATCTGAAACCAGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATC          <  2:427893/150‑1                                                                                                                                      gtgccgGAAACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACG  >  2:329469/7‑150                                                                                                                                                                                                                                                                                             CGCCACCGACACCGTAACGGTGAAAGGGGGCTGGGCGACGGCATTTAAAGCACCTTCTCTGTTGCAACTTAGCCCTGACTGGACGAGCAATTCCTGCCGTGGCGCATGTAAGATTGTGGGTAGCCCGGATCTGAAACCAGAAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2196799‑2196656 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACG  >  REL606/3651091‑3651229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.