| |
| pKD3 |
CamR |
|
Datsenko&Wanner2000 |
Wanner template plasmid |
| pKD13 |
KanR |
|
Datsenko&Wanner2000 |
Wanner template plasmid |
| pCR2.1 |
KanR, AmpR |
|
|
TOPO cloning kit vector |
| pACYC184 |
TetR, CamR |
|
Chang&Cohen1978 |
Lenski evolution experiment |
| pKO3, pKOV |
SucS, CamR |
|
Link1997 |
Church vectors for creating deletions; note: Grow at 30 degrees Celsius |
| pRA300, pRA301, pRA302 |
SpecR |
|
Akakura2002 |
Broad host range, promoterless lacZ map |
Common Plasmid Box
Copy of box resides in –80°C freezer in both Welch and MBB.
| plasmid |
strain |
| pACBSR |
TOP10 |
| pKD3 |
BW25141 |
| pKD13 |
BW2514 |
| pKD32 |
BW2514 |
| pKD46 |
BW25141 |
| pCP20 |
BT340 |
| pRA300 |
? |
| pRA301 |
? |
| pRA302 |
? |
| pACYC184 |
ER2420 |
References
| Akakura2002 |
Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780. |
| Chang&Cohen1978 |
Chang, A. and Cohen, S. (1978) J. Bacteriol., 134: 1141-1156. |
| Datsenko&Wanner2000 |
Datsenko, K.A. and Wanner, B.L. (2000) Proc Natl Acad Sci U S A 97(12):6640–6645. |
| Kitagawa2005 |
Kitagawa, M. et al. (2005) DNA Res. 12: 291-9 |
| Link1997 |
Link, A.J., Phillips, D, and Church, G.M. (1997) J. Bacteriol., 179(20): :6228-37. |
| META FILEATTACHMENT |
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| META FILEATTACHMENT |
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| META FILEATTACHMENT |
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| META FILEATTACHMENT |
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| META FILEATTACHMENT |
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| META FILEATTACHMENT |
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| META FILEATTACHMENT |
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| META FILEATTACHMENT |
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| META FILEATTACHMENT |
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| META FILEATTACHMENT |
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|
