Difference: ReferencePlasmids (14 vs. 15)

Revision 152012-04-04 - JeffreyBarrick

 
META TOPICPARENT name="ReferenceList"

Commonly Used Plasmids

plasmid markers origin ref description
pCA24N CamR   Kitagawa2005 ASKA collection vector
pKD46 AmpR   Datsenko&Wanner2000 Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3 CamR   Datsenko&Wanner2000 Wanner template plasmid
pKD13 KanR   Datsenko&Wanner2000 Wanner template plasmid
pCR2.1 KanR, AmpR     TOPO cloning kit vector
pACYC184 TetR, CamR   Chang&Cohen1978 Lenski evolution experiment
pKO3, pKOV SucS, CamR   Link1997 Church vectors for creating deletions
pRA300, pRA301, pRA302 SpecR   Akakura2002 Broad host range, promoterless lacZ map

Plasmids currently in common plasmid box (located in -80 in both Welch and MBB)

plasmid strain
Changed:
<
<
pACBAR TOP10
>
>
pACBSR TOP10
 
pKD3 BW25141
pKD13 BW2514
pKD32 BW2514
pKD46 BW25141
pCP20 BT340
pRA300 ?
pRA301 ?
pRA302 ?
pACYC184 ER2420

References

Akakura2002 Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780.
Chang&Cohen1978 Chang, A. and Cohen, S. (1978) J. Bacteriol., 134: 1141-1156.
Datsenko&Wanner2000 Datsenko, K.A. and Wanner, B.L. (2000) Proc Natl Acad Sci U S A 97(12):6640–6645.
Kitagawa2005 Kitagawa, M. et al. (2005) DNA Res. 12: 291-9
Link1997 Link, A.J., Phillips, D, and Church, G.M. (1997) J. Bacteriol., 179(20): :6228-37.

META FILEATTACHMENT attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT attachment="pKD46.gb" attr="" comment="lambda red plasmid genbank file" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"
META FILEATTACHMENT attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1"
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