Difference: ReferencePlasmids (11 vs. 12)

Revision 122012-03-27 - CraigBarnhart

 
META TOPICPARENT name="ReferenceList"

Commonly Used Plasmids

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plasmid markers description
pCA24N CamR ASKA collection vector
pKD46 AmpR Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3 CamR Wanner template plasmid
pKD13 KanR Wanner template plasmid
pCR2.1 KanR, AmpR TOPO cloning kit vector
pACYC184 TetR, CamR Lenski evolution experiment
pKO3, pKOV SucS, CamR Church vectors for creating deletions
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plasmid markers description origin instructions reference
pCA24N CamR ASKA collection vector      
pKD46 AmpR Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.      
pKD3 CamR Wanner template plasmid      
pKD13 KanR Wanner template plasmid      
pCR2.1 KanR, AmpR TOPO cloning kit vector      
pACYC184 TetR, CamR Lenski evolution experiment      
pKO3, pKOV SucS, CamR Church vectors for creating deletions      
 
META FILEATTACHMENT attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT attachment="pKD46.gb" attr="" comment="lambda red plasmid genbank file" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"
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