Barrick Lab :: ReferencePlasmids

---+ Commonly Used Plasmids

| *plasmid* | *markers* | *origin* |  *host* | *ref*  | *description* |
| pCA24N | Cam<sup>R</sup> | | | [[http://dnaresearch.oxfordjournals.org/content/12/5/291.long][Kitagawa2005]] | ASKA collection vector |
| pKD46 | Amp<sup>R</sup> | TS | ? | [[http://www.ncbi.nlm.nih.gov/pubmed/10829079][Datsenko&Wanner2000]] | Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number. |
| pKD78 | Cam<sup>R</sup> | TS | | | Identical plasmid to pKD46, but with CAM resistance instead of AMP.|
| pKD3 | Cam<sup>R</sup> | | BW25141 | [[http://www.ncbi.nlm.nih.gov/pubmed/10829079][Datsenko&Wanner2000]] | Wanner template plasmid |
| pKD13 | Kan<sup>R</sup> | | BW25141 | [[http://www.ncbi.nlm.nih.gov/pubmed/10829079][Datsenko&Wanner2000]] | Wanner template plasmid |
| pCR2.1 | Kan<sup>R</sup>, Amp<sup>R</sup> | | | | TOPO cloning kit vector |
| pACYC184 | Tet<sup>R</sup>, Cam<sup>R</sup> | | ER2420 | [[http://jb.asm.org/content/134/3/1141.full.pdf+html][Chang&Cohen1978]] | Lenski evolution experiment |
| pKO3, pKOV | Suc<sup>S</sup>, Cam<sup>R</sup> | | | [[http://www.ncbi.nlm.nih.gov/pubmed/9335267][Link1997]] | Church vectors for creating deletions; note:  Grow at 30° Celsius |
| pACBSR | Cam<sup>R</sup> | p15A | | [[http://www.ncbi.nlm.nih.gov/pubmed/12853150][Herring2003]] | Low copy number. Use a midi or maxiprep. |
| pRA300, pRA301, pRA302 | Spec<sup>R</sup> | | | [[http://www.jbc.org/content/277/18/15773.full.pdf+html][Akakura2002]] | Broad host range, promoterless _lacZ_ [[%ATTACHURLPATH%/pRA_map.png][map]] |
| pCP20 | Amp<sup>R</sup> | TS | BT340 | [[ProcedureFLPFRTRecombination][FLP recombinase protocol]] [[http://msb.embopress.org/content/2/1/2006.0008.long][Baba 2006]] | Temperature sensitive origin, FLP recombinase. Commonly used for removal of KAN cassette from Keio collection. |  

Plasmids listed on this page reside in the common plasmid box in the –80°C freezers in both Welch and MBB labs.

---++ Useful Links
   * [[http://www.qiagen.com/knowledge-and-support/spotlight/plasmid-resource-center/growth%20of%20bacterial%20cultures][Origins of replication and copy numbers of various plasmids and cosmids - Qiagen]]

Attachments

Topic attachments
I	Attachment	History	Action	Size	Date	Who
gbk	pACYC184.gbk	r1	manage	7.7 K	2009-08-03 - 21:09	JeffreyBarrick
gbk	pCA24N.gbk	r1	manage	9.9 K	2009-05-29 - 13:21	JeffreyBarrick
gbk	pCR2.1.gbk	r2 r1	manage	7.1 K	2012-03-07 - 21:11	JeffreyBarrick
gbk	pKD13_Kan_template.gbk	r1	manage	7.6 K	2009-05-29 - 13:22	JeffreyBarrick
gbk	pKD3_Cam_template.gbk	r1	manage	6.6 K	2009-05-29 - 13:22	JeffreyBarrick
gb	pKD46.gb	r1	manage	16.4 K	2012-01-20 - 21:09	BrianRenda
txt	pKOV.txt	r1	manage	10.6 K	2011-02-21 - 17:13	JeffreyBarrick
gbk	pkd4.gbk	r1	manage	6.1 K	2011-12-19 - 22:29	BrianRenda
gbk	pkd4_with_gfp_sites_marked.gbk	r1	manage	10.0 K	2011-12-19 - 22:35	BrianRenda

This topic: Lab > WebHome > ReferenceList > ReferencePlasmids
Topic revision: r22 - 2014-05-12 - DanielDeatherage